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Pregledni rad
https://doi.org/10.2478/v10004-007-0028-0

3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability

Michal Harel
Boris Brumshtein
Ran Meged
Hay Dvir
Raimond B.G. Ravelli
Andrew Mcarthy
Lilly Toker
Israel Silman
Joel L. Sussman

Puni tekst: engleski, pdf (189 KB) str. 347-353 preuzimanja: 955* citiraj
APA 6th Edition
Harel, M., Brumshtein, B., Meged, R., Dvir, H., Ravelli, R.B.G., Mcarthy, A., ... Sussman, J.L. (2007). 3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability. Arhiv za higijenu rada i toksikologiju, 58 (3), 347-353. https://doi.org/10.2478/v10004-007-0028-0
MLA 8th Edition
Harel, Michal, et al. "3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability." Arhiv za higijenu rada i toksikologiju, vol. 58, br. 3, 2007, str. 347-353. https://doi.org/10.2478/v10004-007-0028-0. Citirano 21.11.2019.
Chicago 17th Edition
Harel, Michal, Boris Brumshtein, Ran Meged, Hay Dvir, Raimond B.G. Ravelli, Andrew Mcarthy, Lilly Toker, Israel Silman i Joel L. Sussman. "3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability." Arhiv za higijenu rada i toksikologiju 58, br. 3 (2007): 347-353. https://doi.org/10.2478/v10004-007-0028-0
Harvard
Harel, M., et al. (2007). '3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability', Arhiv za higijenu rada i toksikologiju, 58(3), str. 347-353. https://doi.org/10.2478/v10004-007-0028-0
Vancouver
Harel M, Brumshtein B, Meged R, Dvir H, Ravelli RBG, Mcarthy A i sur. 3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability. Arh Hig Rada Toksikol. [Internet]. 2007 [pristupljeno 21.11.2019.];58(3):347-353. https://doi.org/10.2478/v10004-007-0028-0
IEEE
M. Harel, et al., "3-D Structure of Serum Paraoxonase 1 Sheds Light on Its Activity, Stability, Solubility and Crystallizability", Arhiv za higijenu rada i toksikologiju, vol.58, br. 3, str. 347-353, 2007. [Online]. https://doi.org/10.2478/v10004-007-0028-0

Sažetak
Serum paraoxonases (PONs) exhibit a wide range of physiologically important hydrolytic activities, including drug metabolism and detoxification of nerve gases. PON1 and PON3 reside on high-density lipoprotein (HDL) (the “good cholesterol”), and are involved in the alleviation of atherosclerosis. Members of the PON family have been identified not only in mammals and other vertebrates, but also in invertebrates. We earlier described the first crystal structure of a PON family member, a directly-evolved variant of PON1, at 2.2 Å resolution. PON1 is a 6-bladed beta-propeller with a unique active-site lid which is also involved in binding to HDL. The 3-D structure, taken together with directed evolution studies, permitted analysis of mutations which enhanced the stability, solubility and crystallizability of this PON1 variant. The structure permits a detailed description of PON1’s active site and suggests possible mechanisms for its catalytic activity on certain substrates.

Ključne riječi
catalytic mechanism; crystal-forming variants; directed evolution

Hrčak ID: 16534

URI
https://hrcak.srce.hr/16534

[hrvatski]

Posjeta: 1.323 *