Veterinarski arhiv, Vol. 87 No. 2, 2017.
Izvorni znanstveni članak
The key role of Tumor Necrosis Factor alpha (TNF-α) in vaccinated rainbow trout via irradiated Ichthyophthirius multifiliis trophont
Milad Akbari
; Nuclear science and technology research institute, Karaj, Iran; Department of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Vahid Taghizadeh
; Department of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Marzieh Heidarieh
orcid.org/0000-0003-2097-960X
; Nuclear science and technology research institute, Karaj, Iran
Abdolmajid Hajimoradloo
; Department of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Sažetak
In this study, in order to characterize the immune response against Ichthyophthirius multifiliis (I. multifiliis) in the skin, liver, gills and head kidney of immunized rainbow trout, with two types of killed vaccines (γ-irradiation and formalin inactivation of trophont), the gene expression levels of the pro-inflammatory cytokines tumour necrosis factor-α (TNF-α1 and TNF-α2) were evaluated. The vaccinated fish showed significant protection against I. multifiliis 30 days after the second vaccination. We showed that the pro-inflammatory cytokine, TNF-α1, was expressed in rainbow trout after vaccination, not only in the skin but also in the head kidney and liver, whereas TNF-α2 expression was seen in the liver. Also, parasite-related changes in TNF-α1 expression could be detected only in the gills of fish that were exposed to live I. multifiliis trophonts during this experiment. Finally, according to previous reports and the current study, TNF-α1 could be involved in an immune mechanism that can control I. multifiliis infection in vaccinated rainbow trout.
Ključne riječi
tumor necrosis factor alpha (TNF-α); Ichthyophthirius multifiliis; irradiated vaccine; rainbow trout
Hrčak ID:
178610
URI
Datum izdavanja:
15.3.2017.
Posjeta: 1.327 *