Izvorni znanstveni članak
EVIDENCE FOR CHLORDECONE-STIMULATED OXIDATIVE STRESS IN DIFFERENT TISSUES OF THE CICHLID FISH, Pseudetroplus maculatus (Bloch, 1795)
Kunimmal Poothaadammal Asifa
Kumari Chidambaran Chitra
orcid.org/0000-0002-5090-8709
Sažetak
The present study was designed to assess the effect of chlordecone on
the stimulation of oxidative stress in gill, liver and brain tissues of the
cichlid fish, Pseudetroplus maculatus. Fishes were exposed to chlordecone
at two sublethal concentrations - 3.5 and 7 μg/L - for 24, 72 and 96 h,
maintaining ten animals in each group. Chlordecone treatment did not alter
the body weight of the animal. However, significant increase in the secretion
of mucous and decrease in the weights of gill, brain and hepatosomatic
index were observed at 7 μg/L of chlordecone treatment only after 96
h. Gill tissue showed significant increase in the activities of superoxide
dismutase, catalase and glutathione reductase along with elevated levels
of hydrogen peroxide and lipid peroxidation in concentration and timedependent
manner. This could be the defensive mechanism of gill tissue
to escape the toxic effects of chlordecone. In the liver tissue, superoxide
dismutase activity was increased by 39% at 3.5 μg/L and by 73% at 7 μg/L
of chlordecone treatment. Activities of catalase and glutathione reductase
were decreased 3 to 9 times at 3.5 and 7 μg/L concentrations, respectively,
with concomitant increase in hydrogen peroxide generation (17 to 28
times) and lipid peroxidation (3 to 7 times) at the end of 96 h, which reveals
the failure of hepatic antioxidant system to prevent free radical generation
owing to chlordecone exposure. Activities of all antioxidant enzymes in the
brain were inhibited by 29 to 80% along with the induction of hydrogen
peroxide (13 to 20 times) and lipid peroxidation (6 to 11 times), thereby
indicating imbalance in the antioxidant status. Activities of gill and liver
marker enzyme - alkaline phosphatase - and acetylcholinesterase in brain
were decreased. Therefore, imbalance in the antioxidant defence system as
a result of chlordecone toxicity could lead to susceptible oxidative stress in
various tissues of the fish.
Ključne riječi
Chlordecone; Gill; Liver; Brain; Oxidative stress
Hrčak ID:
182657
URI
Datum izdavanja:
7.6.2017.
Posjeta: 1.110 *