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https://doi.org/10.17113/ftb.55.02.17.4991

Lactobacillus casei Encapsulated in Soy Protein Isolate and Alginate Microparticles Prepared by Spray Drying

Jasmina Hadzieva ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Kristina Mladenovska orcid.org/0000-0003-2503-4699 ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Maja Simonoska Crcarevska ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Marija Glavaš Dodov ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Simona Dimchevska ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Nikola Geškovski orcid.org/0000-0002-2073-5632 ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Anita Grozdanov ; Faculty of Technology and Metallurgy, University ‘Ss Cyril and Methodius’, Ruđer Bošković 26, MK-1000 Skopje, Republic of Macedonia
Emil Popovski ; Institute of Chemistry, Faculty of Natural Sciences and Mathematics, University ‘Ss. Cyril and Methodius’, Arhimedova 5, MK-1000 Skopje, Republic of Macedonia
Gjorgji Petruševski orcid.org/0000-0002-9493-0457 ; Research and Development Unit, Alkaloid AD Skopje, Aleksandar Makedonski 12, MK-1000 Skopje, Republic of Macedonia
Marina Chachorovska ; Research and Development Unit, Alkaloid AD Skopje, Aleksandar Makedonski 12, MK-1000 Skopje, Republic of Macedonia
Tanja Petreska Ivanovska ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Lidija Petruševska-Tozi ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia
Sonja Ugarkovic ; Research and Development Unit, Alkaloid AD Skopje, Aleksandar Makedonski 12, MK-1000 Skopje, Republic of Macedonia
Katerina Goracinova orcid.org/0000-0002-8101-6359 ; Faculty of Pharmacy, University ‘Ss Cyril and Methodius’, Mother Theresa 47, MK-1000 Skopje, Republic of Macedonia

Sažetak

This article presents a novel formulation for preparation of Lactobacillus casei 01 encapsulated in soy protein isolate and alginate microparticles using spray drying method. A response surface methodology was used to optimise the formulation and the central composite face-centered design was applied to study the effects of critical material attributes and process parameters on viability of the probiotic after microencapsulation and in simulated gastrointestinal conditions. Spherical microparticles were produced in high yield (64 %), narrow size distribution (d50=9.7 µm, span=0.47) and favourable mucoadhesive properties, with viability of the probiotic of 11.67, 10.05, 9.47 and 9.20 log CFU/g after microencapsulation, 3 h in simulated gastric and intestinal conditions and four-month cold storage, respectively. Fourier-transform infrared spectroscopy confirmed the probiotic stability after microencapsulation, while differential scanning calorimetry and thermogravimetry pointed to high thermal stability of the soy protein isolate-alginate microparticles with encapsulated probiotic. These favourable properties of the probiotic microparticles make them suitable for incorporation into functional food or pharmaceutical products.

Ključne riječi

Lactobacillus casei 01; alginate; soy protein isolate; microparticles; spray drying; formulation optimisation

Hrčak ID:

183064

URI

https://hrcak.srce.hr/183064

Datum izdavanja:

14.6.2017.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.868 *