Izvorni znanstveni članak
Biochemical properties of some toxic proteins
N. Mujić
; Škola narodnog zdravlja »Andrija Štampar«, Medicinski fakultet Zagreb
Sažetak
The toxic proteins from Vip. ammodytes a. venom can be selectively adsorbed on filter paper fibers, so that antigenic composition of filtrated aqueous solution can be considered as changed. The water insoluble precipitate obtained from Vip. ammodyt. toxin can be purified by reprecipitation from aqueous solution at its isoelectric point. This substance shows the properties of a non-toxic pure protein. The Oudin test gives evidence for the presence of three precipitating antigenic components in the venom of Ammodytes viper. Six or seven protein components in crude venom can be distinguished by their different electrophoretic mobilities. The same value for immune serum has been obtained in the neutralization test of Vip. ammodytes toxin, irrespective of which of the activities of venom, haemolysis or toxicity in mice were used for indicating the endpoint. Thus the haemolytic and toxic effects of the venom can be considered as the properties of a single substance. The toxin-antitoxin precipitation curve was of the floculation type showing a maximum, corresponding to the neutralization point as revealed through the measurement of haemolytic activity of venom and antitoxic potency of antivenin. Six protein components with different electrophoretic mobilities can be distinguished when the venom of the spider Latrodectus tredecimguttatus Rossi is examined by electrophoresis. The immune serum against Latrodectus t. R. toxin was analysed electrophoretically.
Ključne riječi
Hrčak ID:
183134
URI
Datum izdavanja:
19.3.1959.
Posjeta: 772 *