Acta clinica Croatica, Vol. 56. No. 1., 2017.
Izvorni znanstveni članak
https://doi.org/10.20471/acc.2017.56.01.03
Cyclin D1, RET and p27 Expression in Papillary Thyroid Microcarcinoma
Ana Barić
; Clinical Department of Nuclear Medicine, Split University Hospital Center, Split
Vinko Marković
; Clinical Department of Nuclear Medicine, Split University Hospital Center, Split
Davor Eterović
; Clinical Department of Nuclear Medicine, Split University Hospital Center, Split
Vladimir Bedeković
; Clinical Department of ENT, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Mirko Kontić
; Clinical Department of ENT
Ljubica Juretić Kuščić
; Clinical Department of Nuclear Medicine, Split University Hospital Center, Split
Valdi Pešutić Pisac
; Clinical Department of Pathology, Forensic Medicine and Cytology, Split University Hospital Center, Split, Croatia
Ante Punda
; Clinical Department of Nuclear Medicine, Split University Hospital Center, Split
Sažetak
The aim of the study was to determine the influence of RET, p27 and cyclin D1 on regional lymph node metastases in papillary microcarcinoma. The analysis included 70 patients with papillary thyroid microcarcinoma that underwent surgery at Split University Hospital Center between 1999 and 2001. Immunohistochemistry was performed on formalin-fixed, paraffi n-embedded tissue by the RET, p27 and cyclin D1 antibodies. Quantification was based on the intensity and distribution of nuclear staining, dividing tumors into those that showed expression (expressors) and those that showed no expression (non-expressors). Univariate analysis using χ²-test and Fisher exact test was performed with the level of statistical significance set at p<0.05. Th ere was no statistically significant difference in the incidence of metastases according to the expression or non-expression of RET mutation (χ²-test: p=0.459; Fisher exact test: p=0.672). Among 25 cases with cyclin D1 expression, 6 had metastases, whereas only 2 of 45 cases with no cyclin D expression had metastases (χ²-test: p=0.014; Fisher exact test: p=0.021), indicating that the expression of cyclin D1 is not crucial for the development of metastases in lymph nodes. In contrast,analysis of p27 expression showed it to be significantly associated with lymph node metastasis because 3 of 45 patients with p27 expression had metastases, indicating a statistically significant correlation between p27 expression and lymph node metastases (χ²-test: p=0.093; Fisher exact test: p=0.124). This study confirmed the importance of the evaluation of RET, p27 and cyclin D1 expression and demonstrated the validity of their application in the assessment of microcarcinoma behavior.
Ključne riječi
Thyroid cancer, papillary [Supplementary Concept]; Thyroid carcinoma, anaplastic; Cyclin D1; Cyclin-dependent kinase inhibitor p27; RET protein, human
Hrčak ID:
184169
URI
Datum izdavanja:
1.3.2017.
Posjeta: 1.806 *