Kratko priopćenje
The influence of lead ions on the release of acetylcholine
Krista Kostial
; Toxicology Department, Institute of Industrial Hygiene, Zagreb
V.B. Vouk
; Toxicology Department, Institute of Industrial Hygiene, Zagreb
Ljerka Purec
; Toxicology Department, Institute of Industrial Hygiene, Zagreb
Sažetak
To test the effect of lead ions upon the output of acetylcholine perfused preparations of cat's superior cervical ganglion were used. The preganglionic nerve was stimulated with supramaximal shocks at a frequency of 2 per sec for 5 min. Addition of lead nitrate (1,0 microgram/ml) to the perfusion fluid (Ringer-Lockc's solution containing 1 : 100 000 eser in sulphate) reduced the amount of acetylcholine in the perfusate to about 35% of its original value (Fig. 1). With higher concentrations of lead ions even less acetylcholine was released on preganglionic stimulation. The effects were only partly reversible. When nictitating membrane contractions were recorded addition of lead ions lo the perfusion fluid caused complete block of ganglionic transmission (in concentrations from 2.0 to 8,0 micrograms/ml) while stimulating the preganglionic nerve fibres (Fig. 2). The reaction of the nictitating membrane lo stimulation of postganglionic fibres stayed unaltered (Fig. 3). It may be supposed that lead ions have a similar influence on cholinergic nerve endings al the neuromuscular junction in spite of differences between these two structures.
Ključne riječi
Hrčak ID:
186668
URI
Datum izdavanja:
25.10.1954.
Posjeta: 740 *