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Genotyping of the Leptin Receptor Gene in Crna Slavonska Pig – Preliminary Results Suggests New Variants of the Promoter
Polonca Margeta
; Department of Special Zootechnic, Faculty of Agriculture in Osijek, J.J. Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Kristina Gvozdanović
; Department of Special Zootechnic, Faculty of Agriculture in Osijek, J.J. Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Ivona Djurkin Kušec
; Department of Special Zootechnic, Faculty of Agriculture in Osijek, J.J. Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Žarko Radišić
; Department of Special Zootechnic, Faculty of Agriculture in Osijek, J.J. Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Goran Kušec
; Department of Special Zootechnic, Faculty of Agriculture in Osijek, J.J. Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Vladimir Margeta
; Department of Special Zootechnic, Faculty of Agriculture in Osijek, J.J. Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Sažetak
Researches on polymorphisms in the porcine LEPR gene and their association with economic traits were widely performed in the past. Manny polymorphisms in different part of the LEPR gene were described and majority of them was associated with economic traits such as growth and fatness. In present study, LEPR gene in 68 Crna Slavonska pigs was genotyped for HinfI polymorphism in the 3.8kb part of LEPR promoter, for HpaII and RsaI polymorphisms in the intron 4 and for ApeKI polymorphism in the exon 14. Allelic and genotype frequencies on polymorphic sites were calculated. Restriction of the 3.8 kb of the promoter region with HinfI revealed presence of two distinct restriction patterns, which haven’t been described so far. Their exact location and also their potential role in LEPR expression, as well as their impact on important economic traits should be explored in the future. Allelic and genotypic frequencies for other three polymorphic sites studied were more or less comparable with previous findings in the literature.
Ključne riječi
Crna Slavonska pig; LEPR polymorphisms
Hrčak ID:
191845
URI
Datum izdavanja:
18.12.2017.
Posjeta: 666 *