Croatica Chemica Acta, Vol. 57 No. 2, 1984.
Izvorni znanstveni članak
Immobilization of Yeast Alcohol Dehydrogenase on Weakly Basic Anion Exchange Resin Beads
Đurđa Vasić-Rački
; Faculty of Technology, The University of Zagreb, Zagreb, Croatia, Yugoslavia
Sažetak
Yeast alcohol dehydrogenase was immobilized on weakly
basic macroporous anion exchange resin beads Lewatit MP-64.
After the adsorption the enzyme was crosslinked by glutaraldehyde:
The activity of the immobilized enzyme was investigated
in the pH 8.9 recirculation reactor system at 303 K. It was found
that the immobilized enzyme was destabilized upon addition of
semicarbazide hydrochloride to the buffer solution.
A greater amount of protein was attached to the support when
ethanol was present in the enzyme solution, but the activity of
the bound enzyme was lower than in the absence of ethanol.
Ključne riječi
Hrčak ID:
194167
URI
Datum izdavanja:
6.4.1984.
Posjeta: 477 *