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Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid

N. Franjić ; Department of Organic Chemistry and Biochemistry, Ruder Boskovic Institute, Bijenicka 54, 41000 Zagreb, Croatia, Yugoslavia
Ž. Kućan ; Department of Organic Chemistry and Biochemistry, Ruder Boskovic Institute, Bijenicka 54, 41000 Zagreb, Croatia, Yugoslavia

Puni tekst: engleski, pdf (4 MB) str. 417-424 preuzimanja: 39* citiraj
APA 6th Edition
Franjić, N. i Kućan, Ž. (1979). Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid. Croatica Chemica Acta, 52 (4), 417-424. Preuzeto s https://hrcak.srce.hr/195865
MLA 8th Edition
Franjić, N. i Ž. Kućan. "Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid." Croatica Chemica Acta, vol. 52, br. 4, 1979, str. 417-424. https://hrcak.srce.hr/195865. Citirano 26.02.2021.
Chicago 17th Edition
Franjić, N. i Ž. Kućan. "Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid." Croatica Chemica Acta 52, br. 4 (1979): 417-424. https://hrcak.srce.hr/195865
Harvard
Franjić, N., i Kućan, Ž. (1979). 'Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid', Croatica Chemica Acta, 52(4), str. 417-424. Preuzeto s: https://hrcak.srce.hr/195865 (Datum pristupa: 26.02.2021.)
Vancouver
Franjić N, Kućan Ž. Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid. Croatica Chemica Acta [Internet]. 1979 [pristupljeno 26.02.2021.];52(4):417-424. Dostupno na: https://hrcak.srce.hr/195865
IEEE
N. Franjić i Ž. Kućan, "Nucleases in Yeast Acting on the 3'-Terminus of Transfer Ribonucleic Acid", Croatica Chemica Acta, vol.52, br. 4, str. 417-424, 1979. [Online]. Dostupno na: https://hrcak.srce.hr/195865. [Citirano: 26.02.2021.]

Sažetak
Two enzymes capable of hydrolytic removal of 3'-terminal
adenosine from specifically labeled tRNA were identified in yeast.
One of them was highly purified by chromatography on phosphocellulose.
It acts on the 3'-terminus of tRNA by removing free
adenosine, followed by 2'- (or 3'-) cytidylic acid; it also hydrolyses
dinucleoside monophosphates, viral and ribosomal RNA, but it is
inactive against either native or denatured DNA. Hence, the
enzyme is a ribonucleate 3'-nucleotidohydrolase, and may be
identical to previously described »ribosomal« ribonuclease of yeast.
The other enzyme is a labile acidic protein, with M. W. around
31000. It removes the 3'-terminal adenosine of tRNA as adenosine-
5'-monophosphate in a reaction with a pH optimum of 7.2, which
is not inhibited by Mgrt. Chemical and photochemical modification
of tRNA increases its susceptibility to the enzyme. In conjunction
with tRNA adenylyl (cytidylyl) transferase, the enzyme may be
responsible for the turnover of 3'-terminal adenylic acid in yeast.

Hrčak ID: 195865

URI
https://hrcak.srce.hr/195865

Posjeta: 79 *