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https://doi.org/10.15255/KUI.2017.036

Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants

Anita Šalić ; University of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, HR-10 000 Zagreb, Croatia
Ivica Strelec   ORCID icon orcid.org/0000-0003-3999-8499 ; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31 000 Osijek, Croatia
Bruno Zelić   ORCID icon orcid.org/0000-0003-3210-2960 ; University of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, HR-10 000 Zagreb, Croatia
Marina Tišma   ORCID icon orcid.org/0000-0002-8846-3127 ; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31 000 Osijek, Croatia

Puni tekst: engleski, pdf (311 KB) str. 109-116 preuzimanja: 49* citiraj
APA 6th Edition
Šalić, A., Strelec, I., Zelić, B. i Tišma, M. (2018). Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants. Kemija u industriji, 67 (3-4), 109-116. https://doi.org/10.15255/KUI.2017.036
MLA 8th Edition
Šalić, Anita, et al. "Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants." Kemija u industriji, vol. 67, br. 3-4, 2018, str. 109-116. https://doi.org/10.15255/KUI.2017.036. Citirano 20.09.2019.
Chicago 17th Edition
Šalić, Anita, Ivica Strelec, Bruno Zelić i Marina Tišma. "Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants." Kemija u industriji 67, br. 3-4 (2018): 109-116. https://doi.org/10.15255/KUI.2017.036
Harvard
Šalić, A., et al. (2018). 'Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants', Kemija u industriji, 67(3-4), str. 109-116. https://doi.org/10.15255/KUI.2017.036
Vancouver
Šalić A, Strelec I, Zelić B, Tišma M. Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants. Kemija u industriji [Internet]. 2018 [pristupljeno 20.09.2019.];67(3-4):109-116. https://doi.org/10.15255/KUI.2017.036
IEEE
A. Šalić, I. Strelec, B. Zelić i M. Tišma, "Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants", Kemija u industriji, vol.67, br. 3-4, str. 109-116, 2018. [Online]. https://doi.org/10.15255/KUI.2017.036

Sažetak
Phenolic compounds, such as catechol, are released into the environment from a variety of industrial sources and they present a serious ecosystem burden. This work examined the possibility of using partially purified apple polyphenol oxidase (PPO) for bioremediation of phenolic contaminants. In order to optimize process conditions, the optimal pH and temperature for PPO activity were determined, while PPO affinity toward various phenols, as well as the effect of some salts and organic solvents which can be found in wastewaters, was used to confirm applicability of PPO in wastewater treatment. It was found that partially purified apple PPO shows maximal activity at pH 6.8 and 25 °C, but exhibits more than 85 % of its maximal activity in pH range from 5 to 8, and more than 90 % of activity in temperature range from 10 to 50 °C. PPO showed high affinity for various diphenols, but lack of affinity toward monophenols. Sodium tetraborate decahydrate moderately inhibited PPO activity, while exposure of PPO to the presence of organic solvents (φ = 5 %) caused 40 % loss in its activity. Catechol oxidation by PPO performed for just 5 min in a batch reactor at optimal process conditions resulted in 25 % conversion. Based on obtained data, it seems that partially purified apple PPO has reasonable potential in wastewater treatment.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
catechol oxidation; enzymatic wastewater treatment; phenolic contaminants; polyphenol oxidase (PPO)

Hrčak ID: 196016

URI
https://hrcak.srce.hr/196016

[hrvatski]

Posjeta: 150 *