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https://doi.org/10.2478/acph-2018-0026

Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities

AHMED M. GOUDA   ORCID icon orcid.org/0000-0003-4527-8885 ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Umm Al-Qura University, Makkah 21955, Saudi Arabia; Department of Medicinal Chemistry, Faculty of Pharmacy Beni-Suef University, Beni-Suef 62514, Egypt
AHMED H. ABDELAZEEM ; Department of Medicinal Chemistry, Faculty of Pharmacy Beni-Suef University, Beni-Suef 62514, Egypt
ASHRAF N. ABDALLA ; Department of Pharmacology and Toxicology, Faculty of Pharmacy Umm Al-Qura University, Makkah 21955, Saudi Arabia; Department of Pharmacology, Medicinal and Aromatic Plants Research Institute National Center for Research, Khartoum 2404, Sudan
MUHAMMAD AHMED ; Department of Pharmacology and Toxicology, Faculty of Pharmacy Umm Al-Qura University, Makkah 21955, Saudi Arabia

Puni tekst: engleski, pdf (1 MB) str. 251-273 preuzimanja: 85* citiraj
APA 6th Edition
GOUDA, A.M., ABDELAZEEM, A.H., ABDALLA, A.N. i AHMED, M. (2018). Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities. Acta Pharmaceutica, 68 (3), 251-273. https://doi.org/10.2478/acph-2018-0026
MLA 8th Edition
GOUDA, AHMED M., et al. "Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities." Acta Pharmaceutica, vol. 68, br. 3, 2018, str. 251-273. https://doi.org/10.2478/acph-2018-0026. Citirano 16.06.2019.
Chicago 17th Edition
GOUDA, AHMED M., AHMED H. ABDELAZEEM, ASHRAF N. ABDALLA i MUHAMMAD AHMED. "Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities." Acta Pharmaceutica 68, br. 3 (2018): 251-273. https://doi.org/10.2478/acph-2018-0026
Harvard
GOUDA, A.M., et al. (2018). 'Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities', Acta Pharmaceutica, 68(3), str. 251-273. doi: https://doi.org/10.2478/acph-2018-0026
Vancouver
GOUDA AM, ABDELAZEEM AH, ABDALLA AN, AHMED M. Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities. Acta Pharm. [Internet]. 2018 [pristupljeno 16.06.2019.];68(3):251-273. doi: https://doi.org/10.2478/acph-2018-0026
IEEE
A.M. GOUDA, A.H. ABDELAZEEM, A.N. ABDALLA i M. AHMED, "Pyrrolizine-5-carboxamides: Exploring the impact of various substituents on the anti-inflammatory and anticancer activities", Acta Pharmaceutica, vol.68, br. 3, str. 251-273, 2018. [Online]. doi: https://doi.org/10.2478/acph-2018-0026

Sažetak
Towards optimization of the pyrrolizine-5-carboxamide scaffold, a novel series of six derivatives (4a-c and 5a-c) was prepared and evaluated for their anti-inflammatory, analgesic and anticancer activities. The (EZ)-7-cyano-6-((4-hydroxybenzylidene)amino)-N-(p-tolyl)-2,3-dihydro-1H-pyrrolizine-5-carboxamide (4b) and (EZ)-6-((4-chlorobenzylidene)-amino)-7-cyano-N-(p-tolyl)-2,3-dihydro-1H-pyrrolizine-5-carboxamide (5b) bearing the electron donating methyl group showed the highest anti-inflammatory activity while (EZ)-6-((4-chlorobenzylidene)amino)-7-cyano-N-phenyl-2,3-dihydro-1H-pyrrolizine-5-carboxamide (5a) was the most active analgesic agent. Cytotoxicity of the new compounds was evaluated against the MCF-7, A2780 and HT29 cancer cell lines using the MTT assay. Compounds 4b and 5b displayed high anticancer activity with IC50 in the range of 0.30–0.92 µmol L–1 against the three cell lines, while compound (EZ)-N-(4-chlorophenyl)-7-cyano-6-((4-hydroxybenzylidene)-amino)-2,3-dihydro-1H-pyrrolizine-5-carboxamide (4c) was the most active against MCF-7 cells (IC50 = 0.08 µmol L–1). Both the anti-inflammatory and anticancer activities of the new compounds were dependent on the type of substituent on the phenyl rings. Substituents with opposite electronic effects on the two phenyl rings are preferable for high cytotoxicity against the MCF-7 and A2780 cells. COX inhibition was suggested as the molecular mechanism of the anti-inflammatory activity of the new compounds while no clear relationship could be observed between COX inhibition and anticancer activity. Compound 5b, the most active against the three cell lines, induced dose-dependent early apoptosis with 0.1–0.2 % necrosis in MCF-7 cells. New compounds showed promising drug-likeness scores while the docking study revealed high binding affinity to COX-2. Taken together, this study highlighted the significant impact of the substituents on the anti-inflammatory and anticancer activity of pyrrolizine-5-carboxamides, which could help in further optimization to discover good leads for the treatment of cancer and inflammation.

Ključne riječi
pyrrolizine-5-carboxamide; anticancer; anti-inflammatory; COXs; apoptosis; substituent electronic effect

Hrčak ID: 196039

URI
https://hrcak.srce.hr/196039

Posjeta: 190 *