Izlaganje sa skupa

Intracellular Localization and Some Properties of Cytochrome P450 of Rhizopus nigricans

K. Breskvar ; Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Vrazov trg 2, Slovenia, Yugoslavia
T. Hudnik-Plevnik ; Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Vrazov trg 2, Slovenia, Yugoslavia

Sažetak

An attempt was made to localize in Rhizopus nigricans the
enzyme cytochrome P450, which is required for the hydroxylation
of progesterone. Cytochrome P450 was never found in the mitochondrial
fraction but was detected in the fraction sedimenting
at 105000 g. In R. nigricans this fraction does not seem to be
identical with the rat liver microsomal fraction in respect to the
enzyme activities characteristic for microsomes. In the . presence
of progesterone the subcellular fraction containing cytochrome P450
exhibits modified type II spectral change. Upon solubilization of
this fraction with detergent Triton X-100 a significant amount of
the enzyme is converted to its inactive form P420.

Ključne riječi

Hrčak ID:

196360

URI

https://hrcak.srce.hr/196360

Datum izdavanja:

30.3.1977.

Posjeta: 425 *