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ADMET and DMPK, Vol.6 No.1 Ožujak 2018.

Izvorni znanstveni članak
https://doi.org/10.5599/admet.6.1.473

Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches

Susana Amézqueta ; Departament d’Enginyeria Química i Química Analítica and Institute of Biomedicine of the University of Barcelona (IBUB), Universitat de Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain
Anna Maria Bolioli ; Departament d’Enginyeria Química i Química Analítica and Institute of Biomedicine of the University of Barcelona (IBUB), Universitat de Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain
José Luis Beltrán ; Departament d’Enginyeria Química i Química Analítica and Institute of Biomedicine of the University of Barcelona (IBUB), Universitat de Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain
Clara Ràfols ; Departament d’Enginyeria Química i Química Analítica and Institute of Biomedicine of the University of Barcelona (IBUB), Universitat de Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain

Puni tekst: engleski, pdf (931 KB) str. 47-54 preuzimanja: 42* citiraj
APA 6th Edition
Amézqueta, S., Bolioli, A.M., Beltrán, J.L. i Ràfols, C. (2018). Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches. ADMET and DMPK, 6 (1), 47-54. https://doi.org/10.5599/admet.6.1.473
MLA 8th Edition
Amézqueta, Susana, et al. "Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches." ADMET and DMPK, vol. 6, br. 1, 2018, str. 47-54. https://doi.org/10.5599/admet.6.1.473. Citirano 24.10.2018.
Chicago 17th Edition
Amézqueta, Susana, Anna Maria Bolioli, José Luis Beltrán i Clara Ràfols. "Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches." ADMET and DMPK 6, br. 1 (2018): 47-54. https://doi.org/10.5599/admet.6.1.473
Harvard
Amézqueta, S., et al. (2018). 'Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches', ADMET and DMPK, 6(1), str. 47-54. doi: https://doi.org/10.5599/admet.6.1.473
Vancouver
Amézqueta S, Bolioli AM, Beltrán JL, Ràfols C. Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches. ADMET and DMPK [Internet]. 25.03.2018. [pristupljeno 24.10.2018.];6(1):47-54. doi: https://doi.org/10.5599/admet.6.1.473
IEEE
S. Amézqueta, A.M. Bolioli, J.L. Beltrán i C. Ràfols, "Evaluation of the interactions between human serum albumin (HSA) and warfarin or diflunisal by using molecular fluorescence using two approaches", ADMET and DMPK, vol.6, br. 1, str. 47-54, ožujak 2018. [Online]. doi: https://doi.org/10.5599/admet.6.1.473

Sažetak
Serum albumin is the main drug transporter of the bloodstream and contains two main binding sites: Sudlow I or acidic drug binding site, and Sudlow II or benzodiazepine binding site. Warfarin, a well-known anticoagulant drug commonly used in the prevention of thrombosis and thromboembolism, binds to Sudlow I site, whereas non-steroidal antiinflammatory drugs (NSAIDs) such as diflunisal bind preferentially to Sudlow II site. Albumin is a fluorophore that modifies its fluorescence (quenching or enhancement effect) when it is bound to a drug. The application of the double logarithm Stern-Volmer equation allows the calculation of the stoichiometry and the binding constant of the process. This procedure does not consider the possible interferences coming from the fluorescence of the drug though. Another strategy to evaluate the binding constants is to consider the whole spectrum, taking into account all the possible species in equilibrium; in this case we have used an extended version of the STAR program, which can deal with 300 spectra, each containing up to 300 data points. The aim of this work is to compare both approaches to evaluate the interaction between warfarin (Sudlow I) and diflunisal (Sudlow II) and HSA: the double logarithm Stern-Volmer equation and the STAR program.

Ključne riječi
HAS; protein-albumin interaction; fluorescence; warfarin; NSAIDs

Hrčak ID: 196400

URI
https://hrcak.srce.hr/196400

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