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Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning

Steven D. Flanagan ; Department of Biology, Medicine and Psychiatry, University of California, La Jolla, California 92037, USA
Palmer Taylor ; Department of Biology, Medicine and Psychiatry, University of California, La Jolla, California 92037, USA
Samuel H. Barondes ; Department of Biology, Medicine and Psychiatry, University of California, La Jolla, California 92037, USA

Puni tekst: engleski, pdf (10 MB) str. 449-457 preuzimanja: 45* citiraj
APA 6th Edition
Flanagan, S.D., Taylor, P. i Barondes, S.H. (1975). Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning. Croatica Chemica Acta, 47 (3), 449-457. Preuzeto s https://hrcak.srce.hr/196639
MLA 8th Edition
Flanagan, Steven D., et al. "Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning." Croatica Chemica Acta, vol. 47, br. 3, 1975, str. 449-457. https://hrcak.srce.hr/196639. Citirano 08.03.2021.
Chicago 17th Edition
Flanagan, Steven D., Palmer Taylor i Samuel H. Barondes. "Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning." Croatica Chemica Acta 47, br. 3 (1975): 449-457. https://hrcak.srce.hr/196639
Harvard
Flanagan, S.D., Taylor, P., i Barondes, S.H. (1975). 'Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning', Croatica Chemica Acta, 47(3), str. 449-457. Preuzeto s: https://hrcak.srce.hr/196639 (Datum pristupa: 08.03.2021.)
Vancouver
Flanagan SD, Taylor P, Barondes SH. Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning. Croatica Chemica Acta [Internet]. 1975 [pristupljeno 08.03.2021.];47(3):449-457. Dostupno na: https://hrcak.srce.hr/196639
IEEE
S.D. Flanagan, P. Taylor i S.H. Barondes, "Purification of Acetylcholine-Receptor Enriched Membranes by Use of Affinity Partitioning", Croatica Chemica Acta, vol.47, br. 3, str. 449-457, 1975. [Online]. Dostupno na: https://hrcak.srce.hr/196639. [Citirano: 08.03.2021.]

Sažetak
Membrane fragments rich in acetylcholine receptor from the
electric organ of Torpedo caLifornica were purified by a combination
of classical methods and affinity partitioning, a method analogous
to affinity chromatography. Affinity partitioning is based upon the
phase partition method where two water-rich liquid phases are
formed upon the addition of sufficient quantities of water soluble
polymers such as poly(ethylene oxide) and dextran. A phase
system in which less than 2°/o of acetylcholinesterase, adenosine
triphosphatase and a-toxin binding activities di,s,tribute ilnto the
poly(ethylene oxide) rich phase was chosen. By adding cholinergic
derivatives of poly(ethylene oxide), selective changes in the
distribution of membrane fragments rich in acetylcholine receptor
into the poly(ethylene oxide) rich were achieved. Sodium dodecyl
sulfate polyacrylamide gel electrophoresis as well as assay of
cobra a-toxin binding, acetylcholinesterase and adenosinetriphosphatase activities indicate that substantial purification of membrane- bound acetylcholine receptor was achieved.

Hrčak ID: 196639

URI
https://hrcak.srce.hr/196639

Posjeta: 98 *