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https://doi.org/10.15255/CABEQ.2017.1181

Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis

B. Wang ; a) College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China; e) Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou 362000, China
Y. Pu ; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
H. G. Gerken ; Arizona Center for Algal Technology and Innovation, Arizona State University, Mesa, Arizona 85212, USA
Y. Xie ; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China
L. Lin ; College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China
H. Chen ; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Y. Lu ; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Puni tekst: engleski, pdf (969 KB) str. 135-140 preuzimanja: 164* citiraj
APA 6th Edition
Wang, B., Pu, Y., Gerken, H.G., Xie, Y., Lin, L., Chen, H. i Lu, Y. (2018). Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis. Chemical and Biochemical Engineering Quarterly, 32 (1), 135-140. https://doi.org/10.15255/CABEQ.2017.1181
MLA 8th Edition
Wang, B., et al. "Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis." Chemical and Biochemical Engineering Quarterly, vol. 32, br. 1, 2018, str. 135-140. https://doi.org/10.15255/CABEQ.2017.1181. Citirano 01.04.2020.
Chicago 17th Edition
Wang, B., Y. Pu, H. G. Gerken, Y. Xie, L. Lin, H. Chen i Y. Lu. "Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis." Chemical and Biochemical Engineering Quarterly 32, br. 1 (2018): 135-140. https://doi.org/10.15255/CABEQ.2017.1181
Harvard
Wang, B., et al. (2018). 'Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis', Chemical and Biochemical Engineering Quarterly, 32(1), str. 135-140. https://doi.org/10.15255/CABEQ.2017.1181
Vancouver
Wang B, Pu Y, Gerken HG, Xie Y, Lin L, Chen H i sur. Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis. Chemical and Biochemical Engineering Quarterly [Internet]. 2018 [pristupljeno 01.04.2020.];32(1):135-140. https://doi.org/10.15255/CABEQ.2017.1181
IEEE
B. Wang, et al., "Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis", Chemical and Biochemical Engineering Quarterly, vol.32, br. 1, str. 135-140, 2018. [Online]. https://doi.org/10.15255/CABEQ.2017.1181

Sažetak
D-Glyceric acid (D-GA) is a promising chemical for minimizing the toxic effect of acetaldehyde. It has been successfully produced by bioprocesses using glycerol as a substrate. However, high concentrations of glycerol are not beneficial for cell growth. A
two-step culture strategy was employed to deal with the ambivalent culture conditions between the growth of Acetobacter tropicalis and the biosynthesis of D-GA. The first stage focuses on biomass accumulation with low initial glycerol concentration; the second
stage provides D-GA accumulation through whole-cell biocatalysis. Approximately 2-fold of D-GA yield and 4.5-fold of D-GA productivity were gained by the two-step culture strategy compared to the traditional fermentation with 150 g L–1 of glycerol. Our results also showed that A. tropicalis was able to catalyze the conversion of glycerol to D-GA in the presence of up to 10 % methanol. These findings have important implications to enhance D-GA yield by strategy optimization and reduce its cost.






Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
glyceric acid; Acetobacter; two-step culture; biocatalysis; fermentation; cost-effective production

Hrčak ID: 197253

URI
https://hrcak.srce.hr/197253

Posjeta: 289 *