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Original scientific paper
https://doi.org/10.5562/cca3343

The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron

Marko Tomin   ORCID icon orcid.org/0000-0001-6894-6810 ; Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Antonija Tomić ; Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Borislav Kovačević ; Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Sanja Tomić   ORCID icon orcid.org/0000-0002-0550-0878 ; Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia

Fulltext: english, pdf (3 MB) pages 187-193 downloads: 377* cite
APA 6th Edition
Tomin, M., Tomić, A., Kovačević, B. & Tomić, S. (2018). The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron. Croatica Chemica Acta, 91 (2), 187-193. https://doi.org/10.5562/cca3343
MLA 8th Edition
Tomin, Marko, et al. "The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron." Croatica Chemica Acta, vol. 91, no. 2, 2018, pp. 187-193. https://doi.org/10.5562/cca3343. Accessed 25 Feb. 2021.
Chicago 17th Edition
Tomin, Marko, Antonija Tomić, Borislav Kovačević and Sanja Tomić. "The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron." Croatica Chemica Acta 91, no. 2 (2018): 187-193. https://doi.org/10.5562/cca3343
Harvard
Tomin, M., et al. (2018). 'The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron', Croatica Chemica Acta, 91(2), pp. 187-193. https://doi.org/10.5562/cca3343
Vancouver
Tomin M, Tomić A, Kovačević B, Tomić S. The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron. Croatica Chemica Acta [Internet]. 2018 [cited 2021 February 25];91(2):187-193. https://doi.org/10.5562/cca3343
IEEE
M. Tomin, A. Tomić, B. Kovačević and S. Tomić, "The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron", Croatica Chemica Acta, vol.91, no. 2, pp. 187-193, 2018. [Online]. https://doi.org/10.5562/cca3343

Abstracts
Dipeptidyl peptidase III (DPP III) is a zinc-dependent peptidase that cleaves dipeptides off of N-termini of its substrates. Previous studies on human DPP III reveal a reaction mechanism similar to that of thermolysin. Since the active site is conserved within the DPP III family, it is not surprising that the mechanism determined for Bacteroides thetaiotaomicron DPP III (BtDPP III) closely resembles that of hDPP III. However, the hydrogen bond network within the model differs slightly from that in the human ortholog, which results in two proposed pathways. The calculated Gibbs activation energy of 90.1 kJ mol–1 is larger than the one calculated from kinetic data for the preferred substrate Arg2-2-naphthylamide at room temperature (69 kJ mol–1), suggesting the importance of treating the whole DPP III enzyme in the calculations.

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords
dipeptidyl peptidase III; DPP III; peptide hydrolysis; reaction mechanism; Bacteroides thetaiotaomicron

Hrčak ID: 201073

URI
https://hrcak.srce.hr/201073

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