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Croatica Chemica Acta, Vol. 91 No. 2, 2018.

Izvorni znanstveni članak
https://doi.org/10.5562/cca3349

Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli

Sabina Božič Abram ; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Maja Marušič ; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Martina Lenarčič Živković ; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Jasna Brčić ; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Janez Plavec   ORCID icon orcid.org/0000-0003-1570-8602 ; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia

Puni tekst: engleski, pdf (7 MB) str. 163-170 preuzimanja: 122* citiraj
APA 6th Edition
Božič Abram, S., Marušič, M., Lenarčič Živković, M., Brčić, J. i Plavec, J. (2018). Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli. Croatica Chemica Acta, 91 (2), 163-170. https://doi.org/10.5562/cca3349
MLA 8th Edition
Božič Abram, Sabina, et al. "Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli." Croatica Chemica Acta, vol. 91, br. 2, 2018, str. 163-170. https://doi.org/10.5562/cca3349. Citirano 19.02.2019.
Chicago 17th Edition
Božič Abram, Sabina, Maja Marušič, Martina Lenarčič Živković, Jasna Brčić i Janez Plavec. "Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli." Croatica Chemica Acta 91, br. 2 (2018): 163-170. https://doi.org/10.5562/cca3349
Harvard
Božič Abram, S., et al. (2018). 'Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli', Croatica Chemica Acta, 91(2), str. 163-170. doi: https://doi.org/10.5562/cca3349
Vancouver
Božič Abram S, Marušič M, Lenarčič Živković M, Brčić J, Plavec J. Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli. Croatica Chemica Acta [Internet]. 2018 [pristupljeno 19.02.2019.];91(2):163-170. doi: https://doi.org/10.5562/cca3349
IEEE
S. Božič Abram, M. Marušič, M. Lenarčič Živković, J. Brčić i J. Plavec, "Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli", Croatica Chemica Acta, vol.91, br. 2, str. 163-170, 2018. [Online]. doi: https://doi.org/10.5562/cca3349

Sažetak
Herein we show how sequences that can form different non-canonical structures affect gene expression levels when inserted in the core of σ70-dependent promoter, between the −35 and −10 elements recognized by RNA polymerase, in E. coli. We note that influence on level of GFP expression varies considerably depending on introduction of non-canonical structural elements in the antisense and sense strands as well as with their propensities to form G-triplex, G-hairpin, hairpin or G-quadruplex structures. Moreover, the extent of repression of expression does not relate to the in vitro thermal stability in a simple manner. Repression is most likely caused by steric interference rather than improper distance between the −35 and −10 elements. Although properties like thermal stability and topology can be somewhat different under in vivo and in vitro conditions, our results suggest that the extent of expression suppression cannot be dependent solely on thermal stabilities of G-rich structures alone.

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
DNA; non-canonical structures; topology; gene expression modulation; E. coli σ70; GFP expression

Hrčak ID: 202771

URI
https://hrcak.srce.hr/202771

Posjeta: 188 *