Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2017.1161
Cellulase Production by Aspergillus niger ATCC 16888 on Copra Waste from Coconut Milk Process In Layered Packed-bed Bioreactor
T. Chysirichote
; Department of Food Engineering, Faculty of Engineering, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung 1, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Sažetak
Solid-state fermentation (SSF) has been used to produce value-added products from agricultural and food processing wastes, but the majority of SSF production has been conducted on a small scale due to the limitations of heat and mass transfer. This research aimed to reduce the temperature changes in packed-bed bioreactor during the cellulase
fermentation of Aspergillus niger ATCC 16888 on copra waste from coconut milk process. The three configurations of bioreactor used in this study consisted of one- (control), two- and three-layered packed-bed bioreactors. Total volumes of packed-bed bioreactor and bed material were 127 and 57 L, respectively. The sterilized air 30 °C was forced
from the bottom of bioreactor at 0.20 vvm. The highest cellulase production (5.0 ± 0.4 FPU g–1DS) was obtained from the bottom zone of the three-layered bioreactor after fermenting for 1 d. However, the greatest growth was found at the top one, in which a large amount of aerial mycelium was detected.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
cellulase; solid-state fermentation; packed-bed bioreactor; Aspergillus niger
Hrčak ID:
203196
URI
Datum izdavanja:
5.7.2018.
Posjeta: 1.059 *