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Food Technology and Biotechnology, Vol.56 No.2 Lipanj 2018.

Izvorni znanstveni članak
https://doi.org/10.17113/ftb.56.02.18.5349

Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)

Sumedha Arora ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Prashant Mohanpuria ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Gurupkar Singh Sidhu ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Inderjit Singh Yadav ; School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, India
Vandna Kumari ; Botany Department, Punjabi University, IN-147002 Patiala, India

Puni tekst: hrvatski, pdf (6 MB) str. 228-237 preuzimanja: 0* citiraj
APA 6th Edition
Arora, S., Mohanpuria, P., Singh Sidhu, G., Singh Yadav, I. i Kumari, V. (2018). Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco). Food Technology and Biotechnology, 56 (2), 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
MLA 8th Edition
Arora, Sumedha, et al. "Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)." Food Technology and Biotechnology, vol. 56, br. 2, 2018, str. 228-237. https://doi.org/10.17113/ftb.56.02.18.5349. Citirano 21.07.2018.
Chicago 17th Edition
Arora, Sumedha, Prashant Mohanpuria, Gurupkar Singh Sidhu, Inderjit Singh Yadav i Vandna Kumari. "Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)." Food Technology and Biotechnology 56, br. 2 (2018): 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
Harvard
Arora, S., et al. (2018). 'Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)', Food Technology and Biotechnology, 56(2), str. 228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
Vancouver
Arora S, Mohanpuria P, Singh Sidhu G, Singh Yadav I, Kumari V. Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco). Food Technology and Biotechnology [Internet]. 29.06.2018. [pristupljeno 21.07.2018.];56(2):228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
IEEE
S. Arora, P. Mohanpuria, G. Singh Sidhu, I. Singh Yadav i V. Kumari, "Kloniranje i karakterizacija limonoid glukoziltransferaze iz mandarine sorte Kinnow (Citrus reticulata Blanco)", Food Technology and Biotechnology, vol.56, br. 2, str. 228-237, Srpanj 2018. [Online]. doi: https://doi.org/10.17113/ftb.56.02.18.5349
Puni tekst: engleski, pdf (6 MB) str. 228-237 preuzimanja: 0* citiraj
APA 6th Edition
Arora, S., Mohanpuria, P., Singh Sidhu, G., Singh Yadav, I. i Kumari, V. (2018). Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco). Food Technology and Biotechnology, 56 (2), 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
MLA 8th Edition
Arora, Sumedha, et al. "Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)." Food Technology and Biotechnology, vol. 56, br. 2, 2018, str. 228-237. https://doi.org/10.17113/ftb.56.02.18.5349. Citirano 21.07.2018.
Chicago 17th Edition
Arora, Sumedha, Prashant Mohanpuria, Gurupkar Singh Sidhu, Inderjit Singh Yadav i Vandna Kumari. "Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)." Food Technology and Biotechnology 56, br. 2 (2018): 228-237. https://doi.org/10.17113/ftb.56.02.18.5349
Harvard
Arora, S., et al. (2018). 'Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)', Food Technology and Biotechnology, 56(2), str. 228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
Vancouver
Arora S, Mohanpuria P, Singh Sidhu G, Singh Yadav I, Kumari V. Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco). Food Technology and Biotechnology [Internet]. 29.06.2018. [pristupljeno 21.07.2018.];56(2):228-237. doi: https://doi.org/10.17113/ftb.56.02.18.5349
IEEE
S. Arora, P. Mohanpuria, G. Singh Sidhu, I. Singh Yadav i V. Kumari, "Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)", Food Technology and Biotechnology, vol.56, br. 2, str. 228-237, Srpanj 2018. [Online]. doi: https://doi.org/10.17113/ftb.56.02.18.5349

Sažetak
Mandarina sorte Kinnow (Citrus reticulata Blanco) popularna je biljka iz roda citrusa u sjeverozapadnoj Indiji, koja se najviše uzgaja u državi Punjab. Međutim, limonoid aglikoni poput limonina uzrokuju gorak okus, što predstavlja najveći problem u industrijskoj proizvodnji soka od agruma. Iz tkiva mandarine sorte Kinnow izoliran je gen limonoid glukoziltransferaza (LGT) što kodira enzim za odgorčavanje, te je ispitan metabolizam razgradnje limonoida u agrumima. Nakon što je gen identificiran i karakteriziran, njegova cjelokupna sekvencija (1533 bp) deponirana je u bazu Nacionalnog centra za biotehnološke informacije (NCBI). Glukozil transferaza iz vrste Citrus reticulata (CrLGT) nalazi se na zasebnoj grani najveće podgrupe i filogenetski se razlikuje od onih u ostalih sorata mandarina, kao što je C. unshiu, te je jedinstvena po mnogim svojstvima. Ekspresija gena CrLGT na razini transkripcije ispitana je pomoću semikvantitativne metode u različitim tkivima biljke, kao što su mladi list, flavedo, albedo, opna i sjeme, tijekom rane (90 dana nakon cvatnje), srednje rane (150-210 dana nakon cvatnje) i kasne (240 dana nakon cvatnje) faze razvoja ploda, te je potvrđeno da je gen imao najveću ekspresiju u flavedu. Zaključeno je da izolirani gen LGT utječe na metaboličku razgradnju limonoida u agrumima. Pojačana ekspresija ovog gena može ublažiti gorčinu soka od agruma i povećati akumulaciju specifičnih glukozida koji imaju antikancerogena svojstva.

Ključne riječi
mandarina sorte Kinnow, gorčina; limonoid glukoziltransferaza; antikancerogena svojstva; semikvantitativni PCR

Hrčak ID: 203490

URI
https://hrcak.srce.hr/203490

[engleski]

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