Kratko priopćenje
https://doi.org/10.17113/ftb.56.02.18.5393
Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments
Ranko Gacesa
; Department of Chemistry, King’s College London, Franklin-Wilkins Building, Stamford Street, London SE1 9NH, UK
Damir Baranasic
; Department of Genetics, University of Kaiserslautern, Postfach 3049, DE-67653 Kaiserslautern, Germany
Antonio Starcevic
; Centre of Research Excellence for Marine Bioprospecting - BioProCro, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Janko Diminic
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Marino Korlević
; Centre for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, HR- 52210 Rovinj, Croatia
Mirjana Najdek
; Centre for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, HR- 52210 Rovinj, Croatia
Maria Blažina
; Centre for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, HR- 52210 Rovinj, Croatia
Davor Oršolić
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Domagoj Kolesarić
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Paul F. Long
; Department of Chemistry, King’s College London, Franklin-Wilkins Building, Stamford Street, London SE1 9NH, UK
John Cullum
; Department of Genetics, University of Kaiserslautern, Postfach 3049, DE-67653 Kaiserslautern, Germany
Daslav Hranueli
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Sandi Orlic
; Center of Excellence for Science and Technology Integrating Mediterranean Region, Microbial Ecology, HR-10000 Zagreb, Croatia
Jurica Zucko
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Sažetak
Three metagenomic libraries were constructed using surface sediment samples from the northern Adriatic Sea. Two of the samples were taken from a highly polluted and an unpolluted site respectively. The third sample from a polluted site had been enriched using crude oil. The results of the metagenome analyses were incorporated in the REDPET relational database (http://redpet.bioinfo.pbf.hr/REDPET), which was generated using the previously developed MEGGASENSE platform. The database includes taxonomic data to allow the assessment of the biodiversity of metagenomic libraries and a general functional analysis of genes using hidden Markov model (HMM) profiles based on the KEGG database. A set of 22 specialised HMM profiles was developed to detect putative genes for hydrocarbon-degrading enzymes. Use of these profiles showed that the metagenomic library generated after selection on crude oil had enriched genes for aerobic n-alkane degradation. The use of this system for bioprospecting was exemplified using potential alkB and almA genes from this library.
Ključne riječi
oil pollution; n-alkane degradation; database
Hrčak ID:
203496
URI
Datum izdavanja:
29.6.2018.
Posjeta: 1.973 *