Kratko priopćenje

https://doi.org/10.24099/vet.arhiv.170526

Cloning, expression and purification of the outer membrane protein28 of Salmonella enterica serovar Typhimurium for subunit vaccine development - a short communication

Mamta Pandey ; Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India
Mumtesh K. Saxena ; Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India
Anjani Saxena ; Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India
Richa Jha ; College of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India
Sunil K. Rastogi ; Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India
Rajesh Kumar ; Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India

Sažetak

Salmonella Typhimurium, a major gastrointestinal pathogen, poses a global threat to human health. Public health problems associated with this organism have increased to the extent that it has become a major issue. The bacterium is becoming resistant to the commonly available antibiotics, and vaccines also suffer from limitations such as short lived immunity. Therefore, there is an urgent need for the development of an effective vaccine. The outer membrane proteins (Omps) of Salmonella have proven their capability to be developed as a vaccine candidate for prevention of salmonellosis. With this aim, in the present study the Omp28 gene of Salmonella Typhimurium was amplified, cloned and expressed under an IPTG induction system. The recombinant protein thus produced was purified and tested for its antigenicity. The antigenicity of the purified protein was confirmed by western blotting with antiserum raised in rabbit against Omps of S. Typhimurium. The Omp28 gene was amplified as a 330bp product. The expressed protein was found to be of approximately 28kDa and it produced a strong signal in western blot analysis. This study concluded that Omp28 may be proven to be an effective candidate for the development of r-DNA vaccine against salmonellosis.

Ključne riječi

cloning; expression; Omp28; Salomonella Typhimurium; vaccine; Western blot

Hrčak ID:

203994

URI

https://hrcak.srce.hr/203994

Datum izdavanja:

14.7.2018.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.169 *