Izvorni znanstveni članak

https://doi.org/10.11613/BM.2018.030703

Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome

Philipp Scholz ; Institute for Clinical Chemistry, Medical Faculty, University of Cologne, Cologne, Germany
Markus Auler ; Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany
Johannes Ruthard ; Institute for Clinical Chemistry, Medical Faculty, University of Cologne, Cologne, Germany
Bent Brachvogel ; Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany; Department of Pediatrics and Adolescent Medicine, Experimental Neonatology, Medical Faculty, University of Cologne, Cologne, Germany
Andreas R. Klatt ; Institute for Clinical Chemistry, Medical Faculty, University of Cologne, Cologne, Germany
Thomas Streichert ; Institute for Clinical Chemistry, Medical Faculty, University of Cologne, Cologne, Germany

Sažetak

Introduction: Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS)
yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and
to investigate whether AnxA8 antibodies are potential biomarkers for APS.
Materials and methods: We enrolled 22 APS patients and 22 healthy controls in this case-control study. We used sodium dodecyl sulfate polyacrylamide
gel electrophoresis and immunoblot to investigate the presence of AnxA8 antibodies, and we applied enzyme-linked immunosorbent
assay to investigate the presence of cardiolipin (CL) and beta-2-glycoprotein I (ß2GPI) antibodies.
Results: The serum of 9/22 APS patients showed AnxA8 IgG isotype antibody reactivity compared to serum of 2/22 healthy controls (P = 0.034).
When we also included weak immunoblot signals, 12/22 APS patients exhibited AnxA8 IgG isotype antibody reactivity compared to 3/22 healthy
controls (P = 0.005). We also investigated the presence of AnxA8 IgM isotype antibodies in the serum of APS patients but found no statistically significant
difference between the APS patient group and healthy control group (P = 0.500). We further investigated the presence of ß2GPI and CL IgG
and IgM isotype antibodies. AnxA8 IgG isotype antibodies were present in APS patients in a similar frequency as the APS “criteria” antibody against
CL (P = 0.764).
Conclusion: We demonstrated that AnxA8 IgG isotype antibodies are potential biomarkers for the diagnosis of APS.

Ključne riječi

annexin A8; antiphospholipid syndrome; antiphospholipid antibodies

Hrčak ID:

206655

URI

https://hrcak.srce.hr/206655

Datum izdavanja:

15.10.2018.

Posjeta: 562 *