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Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women

Anna Majewska   ORCID icon orcid.org/0000-0001-9036-7452 ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland
Maciej Przybylski ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland
Tomasz Dzieciatkowski ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland
Ewa Romejko-Wolniewicz ; 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Sq. 1/3, 02–015 Warsaw, Poland
Julia Zaręba-Szczudlik ; 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Sq. 1/3, 02–015 Warsaw, Poland
Grazyna Mlynarczyk ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland

Puni tekst: engleski, PDF (257 KB) str. 206-206 preuzimanja: 183* citiraj
APA 6th Edition
Majewska, A., Przybylski, M., Dzieciatkowski, T., Romejko-Wolniewicz, E., Zaręba-Szczudlik, J. i Mlynarczyk, G. (2018). Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women. Acta Dermatovenerologica Croatica, 26 (3), 206-206. Preuzeto s https://hrcak.srce.hr/207006
MLA 8th Edition
Majewska, Anna, et al. "Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women." Acta Dermatovenerologica Croatica, vol. 26, br. 3, 2018, str. 206-206. https://hrcak.srce.hr/207006. Citirano 27.10.2021.
Chicago 17th Edition
Majewska, Anna, Maciej Przybylski, Tomasz Dzieciatkowski, Ewa Romejko-Wolniewicz, Julia Zaręba-Szczudlik i Grazyna Mlynarczyk. "Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women." Acta Dermatovenerologica Croatica 26, br. 3 (2018): 206-206. https://hrcak.srce.hr/207006
Harvard
Majewska, A., et al. (2018). 'Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women', Acta Dermatovenerologica Croatica, 26(3), str. 206-206. Preuzeto s: https://hrcak.srce.hr/207006 (Datum pristupa: 27.10.2021.)
Vancouver
Majewska A, Przybylski M, Dzieciatkowski T, Romejko-Wolniewicz E, Zaręba-Szczudlik J, Mlynarczyk G. Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women. Acta Dermatovenerol Croat. [Internet]. 2018 [pristupljeno 27.10.2021.];26(3):206-206. Dostupno na: https://hrcak.srce.hr/207006
IEEE
A. Majewska, M. Przybylski, T. Dzieciatkowski, E. Romejko-Wolniewicz, J. Zaręba-Szczudlik i G. Mlynarczyk, "Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women", Acta Dermatovenerologica Croatica, vol.26, br. 3, str. 206-206, 2018. [Online]. Dostupno na: https://hrcak.srce.hr/207006. [Citirano: 27.10.2021.]

Sažetak

Diagnosis of genital herpes requires a combination of clinical presentation and laboratory studies. Laboratory diagnostics allow us to clearly establish the etiology (HSV-1 or HSV-2) in order to determine the course of infection and prognosis. Decisive factors in the selection of the appropriate test are: diagnostic goals, patient population, specimen type, and implementation of conditions for the specific method. In total, 187 samples collected during a routine gynecological examination from 120 women were examined for the presence of HSV-1 and HSV-2 in the genital area. Two methods were used to test swabs: cell culture isolation and PCR. HSV-1 was the dominant type of virus in both study groups. The cytopathic effect was observed in 67 (35.8%) cultures with clinical material. HSV-1 and HSV-2 DNA were detected by PCR in 73 (39.0%) cell cultures infected with clinical samples. We did not observe typical, virus related cytopathic changes in 13.7% DNA HSV positive cell cultures, but on the other hand we did not detect viral DNA in 6% of positive cell cultures. High values of the parameters, defining the usefulness of diagnostic tests (sensitivity, specificity, and predictive values) in both groups, are determined by previous viral replication in cell culture.

 

Ključne riječi
cell culture; cytopathic effect; diagnosis; genital herpes; HSV; PCR

Hrčak ID: 207006

URI
https://hrcak.srce.hr/207006

Posjeta: 319 *