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https://doi.org/10.15255/KUI.2018.016

Reproducibility of the Selected Methods for Determination of Phenol in Urine by High Performance Liquid Chromatography

Emir Horozić   ORCID icon orcid.org/0000-0003-0242-3093 ; Faculty of Technology, University of Tuzla, Univerzitetska 8, 75 000 Tuzla, B&H
Aida Begić ; Faculty of Pharmacy, University of Tuzla, Univerzitetska 8, 75 000 Tuzla, B&H
Zahida Ademović   ORCID icon orcid.org/0000-0002-3780-1608 ; Faculty of Technology, University of Tuzla, Univerzitetska 8, 75 000 Tuzla, B&H
Sanja Brekalo Lazarević   ORCID icon orcid.org/0000-0002-5704-6494 ; Faculty of Medicine, University of Tuzla, Univerzitetska 1, 75 000 Tuzla, B&H

Puni tekst: hrvatski, pdf (1 MB) str. P107-P114 preuzimanja: 112* citiraj
APA 6th Edition
Horozić, E., Begić, A., Ademović, Z. i Brekalo Lazarević, S. (2018). Ponovljivost odabranih metoda za određivanje fenola u urinu tekućinskom kromatografijom visoke djelotvornosti. Kemija u industriji, 67 (13 (special issue)), P107-P114. https://doi.org/10.15255/KUI.2018.016
MLA 8th Edition
Horozić, Emir, et al. "Ponovljivost odabranih metoda za određivanje fenola u urinu tekućinskom kromatografijom visoke djelotvornosti." Kemija u industriji, vol. 67, br. 13 (special issue), 2018, str. P107-P114. https://doi.org/10.15255/KUI.2018.016. Citirano 11.07.2020.
Chicago 17th Edition
Horozić, Emir, Aida Begić, Zahida Ademović i Sanja Brekalo Lazarević. "Ponovljivost odabranih metoda za određivanje fenola u urinu tekućinskom kromatografijom visoke djelotvornosti." Kemija u industriji 67, br. 13 (special issue) (2018): P107-P114. https://doi.org/10.15255/KUI.2018.016
Harvard
Horozić, E., et al. (2018). 'Ponovljivost odabranih metoda za određivanje fenola u urinu tekućinskom kromatografijom visoke djelotvornosti', Kemija u industriji, 67(13 (special issue)), str. P107-P114. https://doi.org/10.15255/KUI.2018.016
Vancouver
Horozić E, Begić A, Ademović Z, Brekalo Lazarević S. Ponovljivost odabranih metoda za određivanje fenola u urinu tekućinskom kromatografijom visoke djelotvornosti. Kemija u industriji [Internet]. 2018 [pristupljeno 11.07.2020.];67(13 (special issue)):P107-P114. https://doi.org/10.15255/KUI.2018.016
IEEE
E. Horozić, A. Begić, Z. Ademović i S. Brekalo Lazarević, "Ponovljivost odabranih metoda za određivanje fenola u urinu tekućinskom kromatografijom visoke djelotvornosti", Kemija u industriji, vol.67, br. 13 (special issue), str. P107-P114, 2018. [Online]. https://doi.org/10.15255/KUI.2018.016

Sažetak
Determination of phenol in urine is considered as a suitable biomarker of benzene exposure, which is classified as Group A carcinogen by the International Agency for Cancer Research (IARC) and the Environmental Protection Agency (EPA).
The aim of this work was to test reproducibility of several published high performance liquid chromatography (HPLC) methods for phenol determination in urine samples. Previous methods mostly included additional equipment (e.g. fluorescence detector) or an additional step in a sample preparation protocol (e.g. derivatization or steam distillation) which is time consuming and can also cause the loss of analyte. The method that was chosen in this work showed good reproducibility with certain modifications in chromatographic conditions and sample preparation protocol. Optimisation of the method included changes in wavelength, constituents, and the flow rate of mobile phase, and excluded the addition of internal standard in sample preparation protocol. Mobile phase consisted of distilled water: methanol: glacial acetic acid (65 : 34 : 1 v/v %), and Selectra C18 (4.6 × 250 mm, 5 μm) column was used. Chromatographic conditions included the flow rate of 1 ml min–1, room temperature, wavelength at 218 nm and 270 nm, and an injection volume of 20 µl.
At 270 nm, linearity was shown in the range of concentrations of 1–30 mg l–1 (y = 10290x + 15138; R2 = 0.9932), while the achieved percentage of recovery was between 73.8 % and 111.2 % for the tested concentrations.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
phenol; extraction; urine; HPLC

Hrčak ID: 207036

URI
https://hrcak.srce.hr/207036

[hrvatski]

Posjeta: 178 *