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Izvorni znanstveni članak
Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions
M. Fleš
; Institute of Immunology, Zagreb, Croatia, Yugoslavia
B. Pende
; Institute of Immunology, Zagreb, Croatia, Yugoslavia
| Puni tekst: engleski, pdf (8 MB) | str. 81-87 | citiraj | |
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APA 6th Edition Fleš, M. i Pende, B. (1967). Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions. Croatica Chemica Acta, 39 (2), 81-87. Preuzeto s https://hrcak.srce.hr/208093 MLA 8th Edition Fleš, M. i B. Pende. "Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions." Croatica Chemica Acta, vol. 39, br. 2, 1967, str. 81-87. https://hrcak.srce.hr/208093. Citirano 09.03.2021. Chicago 17th Edition Fleš, M. i B. Pende. "Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions." Croatica Chemica Acta 39, br. 2 (1967): 81-87. https://hrcak.srce.hr/208093 Harvard Fleš, M., i Pende, B. (1967). 'Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions', Croatica Chemica Acta, 39(2), str. 81-87. Preuzeto s: https://hrcak.srce.hr/208093 (Datum pristupa: 09.03.2021.) Vancouver Fleš M, Pende B. Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions. Croatica Chemica Acta [Internet]. 1967 [pristupljeno 09.03.2021.];39(2):81-87. Dostupno na: https://hrcak.srce.hr/208093 IEEE M. Fleš i B. Pende, "Fractionation of Tetanus Toxoid by Sephadex Gel Filtration and Immunochemical Characterization of the Fractions", Croatica Chemica Acta, vol.39, br. 2, str. 81-87, 1967. [Online]. Dostupno na: https://hrcak.srce.hr/208093. [Citirano: 09.03.2021.] |
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Sažetak
Three different ty;pes of tetanus toxoid were fractionated on
Sephadex G - 200 gel. The distribution of the fractions was determined
spectrophotometrically at 280 mfL . Specific activity of the fractions
in vitro was determined by flocculation with standard tetanus antitoxin
and by means of immunodiffusion and immunoelectrophoresis.
In all three types of the toxoid the active component appeared in
the first three fractions ; about 750/o was found in the third fraction.
The purity of this fraction, in terms of Lf/mg. of protein nitrogen
(PN), was increased about twic e in comparison with the starting
material. The highest purity, 2400 Lf/ mg. PN, was achieved in the
third fraction of the toxoid type B-1, which w as p repurified, first
by ultrafiltration of the toxin, then, after detoxication, by fractional
precipitation with ammonium sulfate. This fraction was homogeneous
according to immunodiffusion and immunoelectrophoresis te sts.
It has been concluded that fractional purification of t etanus
toxoid can be effectively achieved by gel filtration on Sephadex
G-200, but the effect of purification highly depends on the purity
of the starting material.
Hrčak ID: 208093
URI
https://hrcak.srce.hr/208093
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