Medica Jadertina, Vol. 48 No. 4, 2018.
Izvorni znanstveni članak
Antimicrobial susceptibility of the most common microorganisms isolated from blood
Sabina Cviljević
orcid.org/0000-0001-7871-2954
; Opća županijska bolnica Požega, Odjel za kliničku mikrobiologiju
Dobrinka Petković
; Opća županijska bolnica Požega, Odjel za infektologiju
Božica Lovrić
orcid.org/0000-0002-2762-6439
; Opća županijska bolnica Požega, Odjel za kontrolu kvalitete
Tihomir Jovanović
; Opća županijska bolnica Požega, Odjel za kontrolu kvalitete
Ljubica Farkaš
; Opća županijska bolnica Požega, Odjel za mikrobiologiju
Sažetak
The aim of this study was to determine the prevalence and antimicrobal susceptibility of the most common microorganisms isolated from the blood in the Pozega-Slavonia County and to compare it with data from Croatia and Europe. Materials and methods: In this retrospective study, we processed 2,536 blood samples taken from January 2015 to December 2016. Blood samples were taken in medical wards, in two vials for blood cultures (aerobic and anaerobic), 10 ml blood in each. Children's blood cultures were taken into one vial, 5 ml of blood. Vials were incubated at 37°C for 7 days in the Bact/Alert 3D system (BioMerieux, Marcy-l'Étoile, France). Identification and antimicrobial testing were performed using commercially available automated Vitek 2 system (BioMerieux, Marcy-l'Étoile, France). Results: Out of the 2,536 submitted blood samples, 89 samples (3.5%) were positive for bacterial growth. Gram-positive bacteria (Staphylococcus spp and Streptococcus spp) were 77% and the gram-negative (Enterobacteriaceae) 22%, and one isolate was Candida albicans (1%). The most commonly isolated organisms were coagulase-negative staphylococci (KNS), 57%. Of the gram negative bacteria, the most common isolate was E. coli (13%). Discussion: Antimicrobial susceptibility of E. coli was the highest to carbapenems, third and fourth generation cephalosporins, co-amoxiclav and ciprofloxacin. Conclusion: Very low prevalence (3.5%) of microorganisms isolated from the blood indicates uncritical blood culture sampling. The most common isolates were KNS (57%), which are normally found on the skin, so before sampling blood cultures, it is essential to disinfect the skin and carefully take the blood sample. These resistance data can serve as a clinical tool in the prescription of empiric antimicrobial therapy.
Ključne riječi
antimicrobial susceptibility; blood stream infection; Enterobacteriaceae
Hrčak ID:
208120
URI
Datum izdavanja:
6.11.2018.
Posjeta: 2.018 *