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URINE CONCENTRATIONS OF HIGH-SENSITIVITY CARDIAC TROPONIN I IN HEALTHY ADULTS – PRELIMINARY REFERENCE INTERVALS

PETRA PERVAN ; Dom zdravlja Zagreb-Centar, Zagreb, Hrvatska
TOMO SVAGUŠA orcid id orcid.org/0000-0002-2036-1239 ; Medicinski fakultet Sveučilišta u Zagrebu, Klinička bolnica Dubrava, Klinika za unutarnje bolesti, Zagreb, Hrvatska
INGRID PRKAČIN orcid id orcid.org/0000-0002-5830-7131 ; Medicinski fakultet Sveučilišta u Zagrebu, Klinička bolnica Merkur, Klinika za unutarnje bolesti, Zagreb, Hrvatska
JURICA VUKOVIĆ ; Klinička bolnica Merkur, Odjel za urologiju, Zagreb, Hrvatska
ANDREA RADELJAK ; Klinička bolnica Merkur, Klinički zavod za medicinsku biokemiju i laboratorijsku medicinu, Referentni centar Ministarstva zdravstva Republike Hrvatske za izradu referentnih vrijednosti u području opće medicinske biokemije, Zagreb
SONJA PERKOV ; Klinička bolnica Merkur, Klinički zavod za medicinsku biokemiju i laboratorijsku medicinu, Referentni centar Ministarstva zdravstva Republike Hrvatske za izradu referentnih vrijednosti u području opće medicinske biokemije, Zagreb


Puni tekst: engleski pdf 90 Kb

str. 461-465

preuzimanja: 275

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Sažetak

Recent studies have shown the presence of troponin molecules in urine, with kidneys considered the main organs of elimination. Availability of the new generation of high-sensitivity assays has enabled detection of low concentrations of circulating cardiac troponins, but the high-sensitivity troponin assays are not designed for analysis of urine specimens. The aim of this study was to establish preliminary reference intervals for the high-sensitivity cardiac troponin I (hs-cTnI) concentrations in urine of healthy adults in Croatia. A total of 60 reference persons were selected (30 males and 30 females) and the concentrations of hs-cTnI in random urine samples were determined on the Abbott Architect i1000SR (Abbott Laboratories) analytical system with chemiluminescent immunochemical method on microparticles (CMIA, ARCHITECT STAT High Sensitive Troponin-I), accredited according to the HR EN ISO 15189:2012 standard (Medical laboratorieds – requirements for quality and competence. Geneva, International Organization for Standardization, 2012). We determined the limit of detection, total laboratory precision, expanded measurement uncertainty and preliminary estimates of the gender-specifi c 99th percentile of the upper reference limit (URL) using nonparametric analysis (methods). The male and female 99th percentile cut-off values were 39.3 and 35.2 pg/mL, respectively. The results of this pilot study suggest that troponin I is removed from the blood by the kidneys and can be determined in the urine with CMIA, ARCHITECT STAT High Sensitive Troponin-I assays. Further research is focused on detailed studies of biochemistry and determination of troponin I in the urine as a new biological marker.

Ključne riječi

troponin I; determination in urine

Hrčak ID:

216412

URI

https://hrcak.srce.hr/216412

Datum izdavanja:

30.1.2019.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.304 *