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https://doi.org/10.5513/JCEA01/20.1.2069

Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry

Jaromír Vašiček   ORCID icon orcid.org/0000-0003-4144-8584 ; Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovak Republic
Andrej Balaži ; Research Institute for Animal Production Nitra, National Agricultural and Food Centre, 951 41 Lužianky, Slovak Republic
Ľubomír Ondruška   ORCID icon orcid.org/0000-0002-8460-3137 ; Research Institute for Animal Production Nitra, National Agricultural and Food Centre, 951 41 Lužianky, Slovak Republic
Vladimir Parkanyi ; Research Institute for Animal Production Nitra, National Agricultural and Food Centre, 951 41 Lužianky, Slovak Republic

Puni tekst: engleski, pdf (298 KB) str. 99-107 preuzimanja: 227* citiraj
APA 6th Edition
Vašiček, J., Balaži, A., Ondruška, Ľ. i Parkanyi, V. (2019). Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry. Journal of Central European Agriculture, 20 (1), 99-107. https://doi.org/10.5513/JCEA01/20.1.2069
MLA 8th Edition
Vašiček, Jaromír, et al. "Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry." Journal of Central European Agriculture, vol. 20, br. 1, 2019, str. 99-107. https://doi.org/10.5513/JCEA01/20.1.2069. Citirano 12.08.2020.
Chicago 17th Edition
Vašiček, Jaromír, Andrej Balaži, Ľubomír Ondruška i Vladimir Parkanyi. "Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry." Journal of Central European Agriculture 20, br. 1 (2019): 99-107. https://doi.org/10.5513/JCEA01/20.1.2069
Harvard
Vašiček, J., et al. (2019). 'Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry', Journal of Central European Agriculture, 20(1), str. 99-107. https://doi.org/10.5513/JCEA01/20.1.2069
Vancouver
Vašiček J, Balaži A, Ondruška Ľ, Parkanyi V. Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry. Journal of Central European Agriculture [Internet]. 2019 [pristupljeno 12.08.2020.];20(1):99-107. https://doi.org/10.5513/JCEA01/20.1.2069
IEEE
J. Vašiček, A. Balaži, Ľ. Ondruška i V. Parkanyi, "Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry", Journal of Central European Agriculture, vol.20, br. 1, str. 99-107, 2019. [Online]. https://doi.org/10.5513/JCEA01/20.1.2069

Sažetak
C-reactive protein (CRP) is an acute phase serum protein secreted by liver hepatocytes. Besides its presence in the serum, CRP was also found on the surface of human leukocytes. However, the binding ability of CRP to rabbit leukocytes has never been previously investigated. The objective of this study was to optimize the detection of rabbit CRP binding leukocytes in order to observe the acute phase of immune response by flow cytometry after Complete Freund´s Adjuvant (CFA) administration. Blood samples were analysed using ELISA assay and flow cytometry immediately before and 2 days after the CFA administration. Significant (P<0.01) increase in the proportion of CRP+ leukocytes (up to 10%) and in their subsets (lymphocytes up to 14% and granulocytes up to 8%) were observed in samples after CFA immunization. ELISA also revealed significantly (P<0.01) higher CRP concentration in rabbit blood plasma after CFA immunization (about 3 mg/L) compared to control samples (about 1.5 mg/L) before immunization. In conclusion, the increase in level of CRP protein during the immune response in rabbits could be measured beside the ELISA also using flow cytometry via CRP binding leukocytes. This novel assay could be therefore successfully applied in further biomedical and veterinary research.

Ključne riječi
C-reactive protein; Complete Freund's adjuvant; ELISA; flow cytometry; rabbit leukocytes

Hrčak ID: 218103

URI
https://hrcak.srce.hr/218103

[slovački]

Posjeta: 357 *