Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/20.1.2008
Distribution of nine viruses in Croatian autochthonous grapevine (Vitis vinifera L.) cultivars from Dalmatian region included in clonal selection
Darko Vončina
; University of Zagreb Faculty of Agriculture, Department of Plant Pathology, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Darko Preiner
; University of Zagreb Faculty of Agriculture, Department of Viticulture and Enology, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Silvio Šimon
; Croatian Center for Agriculture, Food and Rural Affairs, Institute for Viticulture and Enology, Jandrićeva 42, 10000 Zagreb, Croatia
Bogdan Cvjetković
; University of Zagreb Faculty of Agriculture, Department of Agricultural Zoology, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Edi Maletić
; University of Zagreb Faculty of Agriculture, Department of Viticulture and Enology, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Ivan Pejić
; University of Zagreb Faculty of Agriculture, Department of Plant Breeding, Genetics, Biometrics and Experimentation, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Jasminka Karoglan Kontić
; University of Zagreb Faculty of Agriculture, Department of Viticulture and Enology, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Sažetak
The investigation of virus incidence was conducted on 14 autochthonous Croatian grapevine cultivars on vines from 51 vineyards located in the Dalmatian region. A total of 1,116 vines were tested by ELISA (enzyme-linked immunosorbent assay) on nine viruses: Arabis mosaic virus (ArMV), Grapevine fanleaf virus (GFLV), Grapevine fleck virus (GFkV), Grapevine leafroll-associated virus 1, 2, 3 and 7 (GLRaV-1, GLRaV-2, GLRaV-3 and GLRaV-7), Grapevine virus A and B (GVA and GVB). ELISA confirmed presence of eight viruses: GLRaV-3 in 888 vines (79.6%), GVA 685 (61.4%), GLRaV1 455 (40.8%), GFkV 223 (19.9%), GFLV 219 (19.6%), GLRaV-2 46 (4.1%), ArMV 36 (3.2%) and GVB 35 (3.1%). Altogether 93 vines (8.3%) were free of all tested viruses, 168 (15.1%) were infected with a single virus, 368 (32.9%) with two, 314 (28.1%) with three, 127 (11.4%) with four, 43 (3.9%) with five, and 3 (0.3%) vines with six viruses. Symptoms of premature leaf color change and downward curling of leaf blades were common, while abnormal branching, short internodes, double nodes and irregular ripening were detected sporadically. The investigation confirms deteriorated sanitary status of all cultivars except Grk and Plavina and indicates the necessity of intensifying grapevine clonal and sanitary selection programs.
Ključne riječi
autochthonous grapevine cultivars; ELISA; grapevine viruses; symptoms
Hrčak ID:
218115
URI
Datum izdavanja:
19.3.2019.
Posjeta: 1.113 *