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https://doi.org/10.11613/BM.2019.020711

Analytical evaluation of the new Seal Autoanalyzer 3 High Resolution for urinary iodine determination

Valentina Vidranski orcid.org/0000-0003-2938-379X ; Department for Oncology and Nuclear Medicine, University Hospital Center Sestre milosrdnice, Zagreb, Croatia; Faculty of medicine Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Maja Franceschi ; Department for Oncology and Nuclear Medicine, University Hospital Center Sestre milosrdnice, Zagreb, Croatia; Faculty of medicine Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Dražena Krilić ; Department for Oncology and Nuclear Medicine, University Hospital Center Sestre milosrdnice, Zagreb, Croatia
Tomislav Jukić ; Department for Oncology and Nuclear Medicine, University Hospital Center Sestre milosrdnice, Zagreb, Croatia; Faculty of medicine Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Ivan Mihaljević ; Department for Nuclear Medicine and Radiation Protection, University Hospital Center Osijek, Osijek, Croatia; Faculty of medicine Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Zvonko Kusić ; Croatian Academy of Sciences and Arts, Zagreb, Croatia

Sažetak

Introduction: The aim of the study was to evaluate the analytical performance of the new colorimetric, automatic analyser, Seal AutoAnalyzer 3 High Resolution (Seal AA3 HR) (Seal Analytical, Wisconsin, USA) for urinary iodine measurement.
Materials and methods: This study included testing of several analytical features of the method involving: imprecision (within-run %CVr, between- run %CVb and total laboratory precision %CVl), measurement uncertainty, carryover, linearity and method comparison, with 70 urine samples including the measuring range (20 - 700 μg/L).
Results: Within-run, %CVb and %CVl of two control levels were 2.03% and 3.04%, 0.51% and 2.61%, and 2.09% and 4.01%, respectively. Carryover effect was less than 1%. The linearity was good in the range of urinary iodine values between 60 and 500 μg/L (R2 = 0.99). Good agreement of urinary iodine values was found between manual technique and Seal AA3 HR, using Passing-Bablok regression (y = 7.84 (- 3.00 to 15.29) + 0.95 (0.90 to 1.00) x) and Blant-Altman test. Cusum test for linearity indicates that there is no significant deviation from linearity (P > 0.1).
Conclusions: The obtained results proved excellent precision, reproducibility and linearity, comparable to the already used, manual method. The New Seal AA3 HR automatic analyser is acceptable for urinary iodine measurement with very good analytical characteristics and can be used for urinary iodine epidemiological studies of the Croatian population.

Ključne riječi

colorimetry; iodine; method evaluation; urine

Hrčak ID:

221083

URI

https://hrcak.srce.hr/221083

Datum izdavanja:

15.6.2019.

Posjeta: 920 *