Veterinarska stanica, Vol. 48 No. 3, 2017.
Stručni rad
Brucellosis outbreak in sheep and swine in Sisak-Moslavina County – a case report
Željko Cvetnić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Irena Reil
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Maja Zdelar-Tuk
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sanja Duvnjak
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Zvonko Dumbović
; Ministarstvo poljoprivrede Republike Hrvatske - Uprava za veterinarstvo i sigurnost hrane, Zagreb, Hrvatska
Inoslav Brkić
; Zavod za javno zdravstvo Sisačko-moslavačke županije, Sisak, Hrvatska
Željko Mihaljević
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Boris Habrun
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Silvio Špičić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sažetak
This paper describes a brucellosis outbreak caused by Brucella melitensis in a flock of sheep located in the vicinity of Dvor na Uni within Sisak-Moslavina County. Within this outbreak, abortions were recorded in 15 (34.1%) of 44 sheep, with the resultant death of two sheep of this flock. Positive serological reactions were confirmed in 20 (28.6%) sheep by the screening Rose Bengal Test, while a confirmatory test (complement fixation test) confirmed positive reactions in 15 (21.4%) of 70 examined sheep. There were also interesting findings of positive reactions (by immunoenzyme test) in 6 (66.7%) of 9 examined pig blood samples. Negative serological reactions were found in the blood of 20 cattle and 5 humans. Bacteriological testing revealed Brucella sp. in the liver and gastric contents of an aborted foetus, in the liver and uterus of a euthanized sheep, and in the lymph nodes of a pig. Further molecular
identification (Bruce–ladder method) showed that all isolates belonged to B. melitensis. This is the first report of the occurrence of B. melitensis in pigs in Croatia. All measures listed under the “Ordinance on measures for suppression and eradication of sheep and goat brucellosis (B. melitensis)” were implemented in this herd. The systematic implementation of the “Programme for control and eradication of sheep and goat brucellosis (B. melitensis) in the Republic of Croatia” has led to the vast majority of sheep and goat flocks in Croatia receiving the status of being officially free from brucellosis (B. melitensis). It can be concluded that there is a constant risk of the introduction of pathogens into Croatia due to the unfavourable epidemiological situation in border areas, and continuous brucellosis control is still necessary.
Ključne riječi
brucelosis; swine; sheep; Sisak- Moslavina County
Hrčak ID:
222282
URI
Datum izdavanja:
11.6.2017.
Posjeta: 824 *