Veterinarska stanica, Vol. 48 No. 4, 2017.
Pregledni rad
Methods for the isolation and identification of bacteria of the genus Campylobacter - classical and molecular methods (Part I)
Marina Mikulić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Andrea Humski
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Bela Njari
; Veterinarski Fakultet Sveučilišta u Zagrebu, Hrvatska
Dora Stojević
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Luka Jurinović
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Silvio Špičić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sanja Duvnjak
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Željko Cvetnić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sažetak
Bacteria of the genus Campylobacter require microaerophilic conditions for growth. Use of recognized standardized protocols and prescribed highly selective liquid and/ or solid media is appropriate for routine analysis with classical methods. After the growth of characteristic colonies, microscopic, phenotype and biochemical methods, immunoenzyme tests, resistotypization and/ or mass spectrometry are applied to determine the Campylobacter species. Two classical serotyping methods are used to differentiate Campylobacter strains. There are various PCR protocols intended for the detection of Campylobacter genus, species or subspecies from food samples, environment or faeces. The main advantages of PCR methods are their speed, sensitivity and specificity. The deficiency of this method is that it does not differentiate viable from dead cells, therefore new protocols based on RT-PCR, NASBA and QRT-PRC molecular methods are under development.
Ključne riječi
Campylobacter spp.; bacterial isolation; species determination; standardized methods; PCR
Hrčak ID:
222557
URI
Datum izdavanja:
26.7.2017.
Posjeta: 1.580 *