Veterinarski arhiv, Vol. 89 No. 3, 2019.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.0314
Effect of dietary carob wholemeal on blood parameters in weaned pigs
Daniel Špoljarić
; Department of Veterinary Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Dejan Marenčić
; Križevci College of Agriculture, Križevci, Croatia
Maja Benković
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Branimira Špoljarić
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ana Belščak Cvitanović
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Gordan Mršić
; Ministry of the Interior, Zagreb, Croatia
Ksenija Vlahović
; Department of Veterinary Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Maja Popović
; Department of Veterinary Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Siniša Srečec
; Križevci College of Agriculture, Križevci, Croatia
Ivana Stolić
; Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
The aim of this study was to investigate the effect of dietary carob wholemeal (Ceratonia siliqua L.) through monitoring the changes in some blood parameters (RBC, LEU, CD45+, CD4+, CD8+, CD21+) on the growth and development of weaned pigs (Swedish Landrace and Yorkshire cross-breeds). Thirty pigs were divided into two groups. In the control group the pigs were fed with a standard feed mixture. In the second, the experimental group (EXP), the feed mixture was enriched daily with carob wholemeal in a dose of 40 g/ kg diet. Both groups were fed ad libitum. At the end of the 42-day experiment the pigs in the EXP groups were on average 23% heavier. The total sugar content, total phenolic content, flavonoids, anthocyanins, tannins and total antioxidant content of the carob wholemeal was determined. Carob wholemeal contains high carbohydrate content (73%). The total phenolic content value was 9.98 ± 0.18 mg GAE/g. The total flavonoid value was 6.56 ± 0.19 mg GAE/g, or 65% of total polyphenols, from which it can be seen that antioxidant activity is correlated to the total phenolic content. The total anthocyanins content was 34.00 ± 0.07 mg CE/g while the total tannin content was 658.75 ± 18.75 μg TAE/g. The ABTS method values were 89.50 ± 0.17 μmol TE/g sample while antioxidant activity determined by the DPPH method was 56.87 ± 0.66 μmol TE/g sample. Carobsupplementation did not affect the amount of red blood cells and leucocytes, but did affect the proportions of the proliferation rate of CD45+ lymphoid cells, CD4+ and CD8+ T cells and CD21+ B cells in peripheral blood. Between days 14 and 42 an increase in the proportions of CD45+, CD4+, CD8+, CD21+ cells (at p≤0.05 or p≤0.01, respectively) was observed. Dietary supplementation of weaned piglets with 4% carob wholemeal showed an advantageous or beneficial effect on the immunity and productivity of the weanlings.
Ključne riječi
carob; diarrhoea; phytocompounds; immunology; pigs
Hrčak ID:
222712
URI
Datum izdavanja:
12.7.2019.
Posjeta: 972 *