Veterinarska stanica, Vol. 49 No. 2, 2018.
Izvorni znanstveni članak
Survey of chloramphenicol residues in meat and meat products during the period 2015 - 2017
Nina Bilandžić
orcid.org/0000-0002-0009-5367
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Petra Vrkljan
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Hrvatska
Božica Solomun Kolanović
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Hrvatska
Ivana Varenina
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Marina Krpan
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Hrvatska
Mirjana Hruškar
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Hrvatska
Sažetak
Despite the ban on the use of chloramphenicol in veterinary medicine in animals intended for nutrition within the European Union (EU) for more than two decades, its residues are systematically determined in all types of animal products. The purpose of this study was to determine chloramphenicol concentrations in meat and meat products using the ELISA method. Method validation was performed according to Commission Decision 2002/657/EC. The following parameters for meat and products were determined: detection capability (CCβ) 0.05 and 0.13 μg/kg; recovery 80.8 and 100%; coefficient of variation 13 and 14.2%; detection limit (LOD) 0.005 and 0.019 μg/kg; quantification limit (LOQ) 0.010 and 0.068 μg/kg. The obtained validation parameters were in accordance with the criteria of the Commission Decision, and the method is suitable for the quantification of chloramphenicol in meat and meat products. In this study, chloramphenicol concentrations were determined in beef and pork meat and various meat products collected during 2015, 2016 and 2017. In a total of 392 samples, chloramphenicol concentrations ranged from 0.0003 to 0.22 μg/kg. The highest value of 0.22 μg/kg was measured in luncheon meat. Mean chloramphenicol values for all tested meat and meat products ranged from 0.0076 to 0.034 μg/kg. No values were found to exceed the prescribed minimum required performance
Food Technology and Biotechnology, University
limit (MRPL; 0.3 μg/kg) for the method used for chloramphenicol quantification. According to the data of the European Food Safety Agency, based on national monitoring plans of chloramphenicol residues in EU Member States for the period 2013–2015, a total of 50 samples of different animal species and products were determined to have elevated chloramphenicol concentrations. The highest number of non-compliant results was recorded in beef and pork meat. In 2015, two non-compliant results of chloramphenicol were found in Croatia, one in beef and the other in poultry meat. In the period from 2008 to 2017, a total of 108 different products with non-compliant chloramphenicol concentrations were reported in the Rapid Alert System for Food and Feed (RASFF) for non-compliant products intended for the EU market. Among those non-compliant samples were 18 meat products, of which 14 were offal of different animal species, three were rabbit meat and one poultry meat. The results of this study showed that the analysed meat products are suitable for human consumption with regard to the measured chloramphenicol residues. However, given the non-compliant results at the EU level, control of these residues is essential at all levels of animal food production and, in particular, control of import consignments of different products.
Ključne riječi
chloramphenicol; meat; meat products; food safety; ELISA; validation
Hrčak ID:
222805
URI
Datum izdavanja:
2.4.2018.
Posjeta: 766 *