Izvorni znanstveni članak
https://doi.org/10.17113/ftb.57.02.19.5832
Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite: Improvement of Loading Amount and Catalytic Activity
Junhui Rong
; School of Chemistry and Chemical Engineering, Jiangsu University, 212013 Zhenjiang, PR China
Zijuan Zhou
; School of Food Science and Nutrition, University of Leeds, Leeds, LS2 9JT, UK
Yun Wang
; School of Chemistry and Chemical Engineering, Jiangsu University, 212013 Zhenjiang, PR China
Juan Han
; School of Food and Biological Engineering, Jiangsu University, 212013 Zhenjiang, PR China
Chunmei Li
; School of Chemistry and Chemical Engineering, Jiangsu University, 212013 Zhenjiang, PR China
Wenli Zhang
; School of Chemistry and Chemical Engineering, Jiangsu University, 212013 Zhenjiang, PR China
Liang Ni
; School of Chemistry and Chemical Engineering, Jiangsu University, 212013 Zhenjiang, PR China
Sažetak
In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe3O4@6arm-PEG-NH2) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34 mg/g) due to the surface of carrier material containing a large amount of amino groups from 6arm-PEG-NH2, but degradation rate of phenols was also much higher (95.4 %), which is attributed to the synergistic effect between the free HRP (45.4 %) and the support material of GO@Fe3O4@6arm-PEG-NH2 (13.6 %). Compared with the free enzyme, thermal, storage and operational stability of the immobilized HRP improved. The immobilized HRP still retained over 68.1 % activity after being reused 8 times. These results suggest that the multi-armed magnetic composite has good application prospect for enzyme immobilization.
Ključne riječi
immobilized enzyme; graphene oxide; multi-armed polymer; horseradish peroxidase; synergistic effect
Hrčak ID:
223880
URI
Datum izdavanja:
30.6.2019.
Posjeta: 1.443 *