hrcak mascot   Srce   HID

Original scientific paper
https://doi.org/10.20471/acc.2019.58.02.18

Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer

Mateusz Rubinkiewicz ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland
Izabela Siemińska ; Department of Clinical Immunology, Institute of Pediatrics, Jagiellonian University Medical College, Kraków, Poland
Piotr Małczak ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland
Piotr Major ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland
Jarosław Baran ; Department of Clinical Immunology, Institute of Pediatrics, Jagiellonian University Medical College, Kraków, Poland
Andrzej Budzyński ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland
Michał Pędziwiatr   ORCID icon orcid.org/0000-0001-7087-6639 ; 2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland; Centre for Research, Training and Innovation in Surgery (CERT AIN Surgery), Krakow, Poland

Fulltext: english, pdf (668 KB) pages 337-342 downloads: 131* cite
APA 6th Edition
Rubinkiewicz, M., Siemińska, I., Małczak, P., Major, P., Baran, J., Budzyński, A. & Pędziwiatr, M. (2019). Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer. Acta clinica Croatica, 58 (2.), 337-342. https://doi.org/10.20471/acc.2019.58.02.18
MLA 8th Edition
Rubinkiewicz, Mateusz, et al. "Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer." Acta clinica Croatica, vol. 58, no. 2., 2019, pp. 337-342. https://doi.org/10.20471/acc.2019.58.02.18. Accessed 19 Oct. 2020.
Chicago 17th Edition
Rubinkiewicz, Mateusz, Izabela Siemińska, Piotr Małczak, Piotr Major, Jarosław Baran, Andrzej Budzyński and Michał Pędziwiatr. "Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer." Acta clinica Croatica 58, no. 2. (2019): 337-342. https://doi.org/10.20471/acc.2019.58.02.18
Harvard
Rubinkiewicz, M., et al. (2019). 'Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer', Acta clinica Croatica, 58(2.), pp. 337-342. https://doi.org/10.20471/acc.2019.58.02.18
Vancouver
Rubinkiewicz M, Siemińska I, Małczak P, Major P, Baran J, Budzyński A, et al. Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer. Acta clinica Croatica [Internet]. 2019 [cited 2020 October 19];58(2.):337-342. https://doi.org/10.20471/acc.2019.58.02.18
IEEE
M. Rubinkiewicz, et al., "Perioperative Changes in Lymphocyte Subpopulations in Patients Undergoing Surgery for Colorectal Cancer", Acta clinica Croatica, vol.58, no. 2., pp. 337-342, 2019. [Online]. https://doi.org/10.20471/acc.2019.58.02.18

Abstracts
Surgical procedure has immense impact on the immune balance. However, little is known about perioperative changes in T regulatory and Th17 lymphocyte subpopulations in patients undergoing colorectal resection. Patients with resectable colon cancer were enrolled in the study. Blood samples were obtained on two occasions, i.e. before the procedure and two days after the surgery. We also recruited a control group of young, healthy individuals. Lymphocyte subpopulations were analyzed with the use of flow cytometry. Investigated subpopulations consisted of total lymphocyte count, CD4+, CD8+, T regulatory Foxp3+ (Tregs), Th17 lymphocytes and white blood cell (WBC) count. There were significant differences in immune cell levels before and after the surgery. Reduction was recorded in the CD4+, CD8+, Tregs and total lymphocyte counts (p=0.002, p=0.01, p=0.008 and p=0.001, respectively). Increase was observed in total WBC and Th17 cells, however, Th17 lymphocytes did not reach statistical significance (p=0.01 and p=0.5, respectively). In conclusion, surgical intervention caused changes in all lymphocyte subpopulations investigated in patients undergoing surgery for colorectal cancer. However, it seemed to be an effect of perioperative trauma. Further studies are needed to investigate the impact of surgical intervention on lymphocyte subpopulations.

Keywords
Lymphocyte subsets; T-lymphocytes, regulatory; Th17 cells; Lymphocyte count; Colorectal surgery

Hrčak ID: 224608

URI
https://hrcak.srce.hr/224608

[croatian]

Visits: 306 *