hrcak mascot   Srce   HID

Original scientific paper
https://doi.org/10.20471/acc.2019.58.02.19

OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor

Nikola Knežević ; Department of Urology, Zagreb University Hospital Centre and School of Medicine, University of Zagreb, Zagreb, Croatia
Tomislav Kuliš   ORCID icon orcid.org/0000-0002-0895-5691 ; Department of Urology, Zagreb University Hospital Centre and School of Medicine, University of Zagreb, Zagreb, Croatia
Luka Penezić   ORCID icon orcid.org/0000-0003-2842-553X ; Department of Urology, Zagreb University Hospital Centre and School of Medicine, University of Zagreb, Zagreb, Croatia
Marijana Ćorić ; Department of Pathology and Cytology, Zagreb University Hospital Centre and School of Medicine, University of Zagreb, Zagreb, Croatia
Ivan Krhen ; Department of Urology, Zagreb University Hospital Centre and School of Medicine, University of Zagreb, Zagreb, Croatia
Željko Kaštelan ; Department of Urology, Zagreb University Hospital Centre and School of Medicine, University of Zagreb, Zagreb, Croatia

Fulltext: english, pdf (391 KB) pages 343-347 downloads: 129* cite
APA 6th Edition
Knežević, N., Kuliš, T., Penezić, L., Ćorić, M., Krhen, I. & Kaštelan, Ž. (2019). OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor. Acta clinica Croatica, 58 (2.), 343-347. https://doi.org/10.20471/acc.2019.58.02.19
MLA 8th Edition
Knežević, Nikola, et al. "OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor." Acta clinica Croatica, vol. 58, no. 2., 2019, pp. 343-347. https://doi.org/10.20471/acc.2019.58.02.19. Accessed 24 Oct. 2020.
Chicago 17th Edition
Knežević, Nikola, Tomislav Kuliš, Luka Penezić, Marijana Ćorić, Ivan Krhen and Željko Kaštelan. "OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor." Acta clinica Croatica 58, no. 2. (2019): 343-347. https://doi.org/10.20471/acc.2019.58.02.19
Harvard
Knežević, N., et al. (2019). 'OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor', Acta clinica Croatica, 58(2.), pp. 343-347. https://doi.org/10.20471/acc.2019.58.02.19
Vancouver
Knežević N, Kuliš T, Penezić L, Ćorić M, Krhen I, Kaštelan Ž. OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor. Acta clinica Croatica [Internet]. 2019 [cited 2020 October 24];58(2.):343-347. https://doi.org/10.20471/acc.2019.58.02.19
IEEE
N. Knežević, T. Kuliš, L. Penezić, M. Ćorić, I. Krhen and Ž. Kaštelan, "OCT4 Immunohistochemistry after Staging Laparoscopic Retroperitoneal Lymphadenectomy for Testicular Tumor", Acta clinica Croatica, vol.58, no. 2., pp. 343-347, 2019. [Online]. https://doi.org/10.20471/acc.2019.58.02.19

Abstracts
Twenty to thirty percent of patients with clinical stage I testicular tumor have metastases in the retroperitoneum. The aim of this study was to evaluate the role of OCT4 immunohistochemistry in histopathologic diagnosis of lymph node metastases in patients with nonseminomatous
germ cell testicular tumors. All clinical stage I patients with staging laparoscopic retroperitoneal lymphadenectomy from 2001 until 2009 were included. Archived materials of dissected lymph nodes were reassessed and additional immunohistochemical staining with OCT4 antibody was performed in patients diagnosed as free from metastases. Each slide was visually estimated for the percentage of tumor cells showing nuclear immunoreactivity for OCT4. The study included 93 patients, of which 30 (32.3%) had initially positive retroperitoneal lymph nodes. Of the remaining 63 patients, materials were missing for 5 patients, so additional immunohistochemical staining was performed in 58 patients. Of these, two (3.4%) patients were OCT4 positive, suggesting a conclusion that they were initially misdiagnosed as stage I and metastasis free. OCT4 proved its value in detecting retroperitoneal
metastases. Staging laparoscopic retroperitoneal lymphadenectomy for nonseminomatous germ cell testicular tumors in clinical stage I is a reasonable option for selected patients.

Keywords
Laparoscopy; Lymphatic metastasis; Lymph node excision; Staining and labeling; Testicular neoplasms

Hrčak ID: 224609

URI
https://hrcak.srce.hr/224609

[croatian]

Visits: 304 *