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COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS

Marijana Skific   ORCID icon orcid.org/0000-0003-3083-6506 ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia; University of Applied Health Sciences, Zagreb, Croatia
Mirna Golemovic ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia; University of Applied Health Sciences, Zagreb, Croatia

Puni tekst: engleski, pdf (861 KB) str. 51-56 preuzimanja: 196* citiraj
APA 6th Edition
Skific, M. i Golemovic, M. (2019). COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS. Molecular and Experimental Biology in Medicine, 2 (2), 51-56. Preuzeto s https://hrcak.srce.hr/226238
MLA 8th Edition
Skific, Marijana i Mirna Golemovic. "COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS." Molecular and Experimental Biology in Medicine, vol. 2, br. 2, 2019, str. 51-56. https://hrcak.srce.hr/226238. Citirano 27.09.2020.
Chicago 17th Edition
Skific, Marijana i Mirna Golemovic. "COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS." Molecular and Experimental Biology in Medicine 2, br. 2 (2019): 51-56. https://hrcak.srce.hr/226238
Harvard
Skific, M., i Golemovic, M. (2019). 'COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS', Molecular and Experimental Biology in Medicine, 2(2), str. 51-56. Preuzeto s: https://hrcak.srce.hr/226238 (Datum pristupa: 27.09.2020.)
Vancouver
Skific M, Golemovic M. COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS. Molecular and Experimental Biology in Medicine [Internet]. 2019 [pristupljeno 27.09.2020.];2(2):51-56. Dostupno na: https://hrcak.srce.hr/226238
IEEE
M. Skific i M. Golemovic, "COLONY-FORMING UNIT ASSAY AS A POTENCY TEST FOR HEMATOPOIETIC STEM/PROGENITOR CELL PRODUCTS", Molecular and Experimental Biology in Medicine, vol.2, br. 2, str. 51-56, 2019. [Online]. Dostupno na: https://hrcak.srce.hr/226238. [Citirano: 27.09.2020.]

Sažetak
Colony-forming unit (CFU) assay is a short-term culture assay used for the detection of functionally active hematopoietic progenitor cells (HPCs) in the in vitro setting. This potency assay enables the identification of colony-producing HPCs in any type of hematopoietic stem cell/progenitor product (HSC/P) including bone marrow (BM), cord blood (CB) and mobilized peripheral blood (MPB). It has been shown that the frequency of HPCs in BM, MPB and CB cellular products directly correlates with the engraftment of both neutrophils and platelets as well as with the overall survival of recipients following hematopoietic stem cell transplantation which makes the CFU assay a good quality parameter for the prediction of engraftment success. The aim of this article is to provide an overview of different approaches to setting up the CFU assay with an emphasis on different sample preparation techniques, cell plating density and colony identification and enumeration used by different laboratories.

Ključne riječi
CFU assay; hematopoietic progenitor cell; potency test; bone marrow; mobilized peripheral blood; cord blood; CFU-GM; BFU-E; CFU-GEMM

Hrčak ID: 226238

URI
https://hrcak.srce.hr/226238

Posjeta: 246 *