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Original scientific paper
https://doi.org/10.21860/medflum2019_227128

Antioxidant activity and kinetic analysis of tocopherol extract obtained from different types of vegetable oils

Paola Tijan ; Sveučilište u Rijeci, Medicinski fakultet, Rijeka, Hrvatska
Dalibor Broznić ; Sveučilište u Rijeci, Medicinski fakultet, Zavod za medicinsku kemiju, biokemiju i kliničku kemiju, Rijeka, Hrvatska

Fulltext: croatian, pdf (2 MB) pages 350-360 downloads: 141* cite
APA 6th Edition
Tijan, P. & Broznić, D. (2019). Antioksidativna aktivnost i kinetička analiza tokoferolnog ekstrakta iz različitih vrsta biljnih ulja. Medicina Fluminensis, 55 (4), 350-360. https://doi.org/10.21860/medflum2019_227128
MLA 8th Edition
Tijan, Paola and Dalibor Broznić. "Antioksidativna aktivnost i kinetička analiza tokoferolnog ekstrakta iz različitih vrsta biljnih ulja." Medicina Fluminensis, vol. 55, no. 4, 2019, pp. 350-360. https://doi.org/10.21860/medflum2019_227128. Accessed 13 Aug. 2020.
Chicago 17th Edition
Tijan, Paola and Dalibor Broznić. "Antioksidativna aktivnost i kinetička analiza tokoferolnog ekstrakta iz različitih vrsta biljnih ulja." Medicina Fluminensis 55, no. 4 (2019): 350-360. https://doi.org/10.21860/medflum2019_227128
Harvard
Tijan, P., and Broznić, D. (2019). 'Antioksidativna aktivnost i kinetička analiza tokoferolnog ekstrakta iz različitih vrsta biljnih ulja', Medicina Fluminensis, 55(4), pp. 350-360. https://doi.org/10.21860/medflum2019_227128
Vancouver
Tijan P, Broznić D. Antioksidativna aktivnost i kinetička analiza tokoferolnog ekstrakta iz različitih vrsta biljnih ulja. Medicina Fluminensis [Internet]. 2019 [cited 2020 August 13];55(4):350-360. https://doi.org/10.21860/medflum2019_227128
IEEE
P. Tijan and D. Broznić, "Antioksidativna aktivnost i kinetička analiza tokoferolnog ekstrakta iz različitih vrsta biljnih ulja", Medicina Fluminensis, vol.55, no. 4, pp. 350-360, 2019. [Online]. https://doi.org/10.21860/medflum2019_227128

Abstracts
Aim: To determine the difference in the antioxidant activity of the olive, sunflower, pumpkin and black oil and their tocopherol extracts as well as to examine the kinetics and mechanism of reactions between free radicals and antioxidants. Materials and Methods: Antioxidant activity of the oils and tocopherol extracts was measured spectrophotometrically using DPPH˙ (2,2-diphenyl-1-picrylhydrazil) while Trolox equivalent was used as a reference standard for the quantification of the results obtained by the DPPH˙ test. DPPH˙ signal inhibition was evaluated and analyzed by monophasic and biphasic kinetics mathematical models. Results: The TEAC values suggest that olive and sunflower oil (2.16 mmol/L) have a stronger antioxidant activity than black (1.85 mmol/L) and pumpkin oil (1.68 mmol/L) and they possess stronger inhibition of the DPPH˙ radical. Tocopherol extracts have a much weaker antioxidant effect than the analyzed vegetable oils, where the sunflower extract is most effective, making 22.1 % of the antioxidant oil activity, while the weakest extract is the pumpkin oil extract oil with only 13.9 %. DPPH˙ radical disappearance in oil samples takes place in two successive phases, the initial exponential which takes place within 7 min of the reaction, depending on the oil type, and the second slow phase where the changes are proportional to the time. The rapid reaction phase by tocopherol extracts was not noticed, indicating that the reaction takes places by the mechanism of the first order kinetics. Conclusion: The results indicate that vegetable oils have a stronger effect on the radical inhibition than the tocopherol extracts, and by their proper consumption they will support the organism in the “fight” against free radicals. In addition, the most important role of the antioxidant defense system against free radicals makes the synergic interactions among the different molecules present in the food, indicating that antioxidants in synergy have a stronger potential than the sum of all individual antioxidants.

Keywords
antioxidant activity; DPPH˙; kinetic analysis; TEAC; tocopherols; vegetable oils

Hrčak ID: 227128

URI
https://hrcak.srce.hr/227128

[croatian]

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