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Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2019-70-3322

Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells

Teuta Murati ; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Marina Miletić ; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Josipa Kolarić ; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Vanja Lovrić ; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Danijela Bursać Kovačević ; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Predrag Putnik ; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Irena Landeka Jurčević ; Laboratory for Food Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Domagoj Đikić ; Division of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Verica Dragović-Uzelac ; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Ivana Kmetič ; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (633 KB) str. 303-308 preuzimanja: 45* citiraj
APA 6th Edition
Murati, T., Miletić, M., Kolarić, J., Lovrić, V., Bursać Kovačević, D., Putnik, P., ... Kmetič, I. (2019). Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells. Arhiv za higijenu rada i toksikologiju, 70 (4), 303-308. https://doi.org/10.2478/aiht-2019-70-3322
MLA 8th Edition
Murati, Teuta, et al. "Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells." Arhiv za higijenu rada i toksikologiju, vol. 70, br. 4, 2019, str. 303-308. https://doi.org/10.2478/aiht-2019-70-3322. Citirano 15.08.2020.
Chicago 17th Edition
Murati, Teuta, Marina Miletić, Josipa Kolarić, Vanja Lovrić, Danijela Bursać Kovačević, Predrag Putnik, Irena Landeka Jurčević, Domagoj Đikić, Verica Dragović-Uzelac i Ivana Kmetič. "Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells." Arhiv za higijenu rada i toksikologiju 70, br. 4 (2019): 303-308. https://doi.org/10.2478/aiht-2019-70-3322
Harvard
Murati, T., et al. (2019). 'Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells', Arhiv za higijenu rada i toksikologiju, 70(4), str. 303-308. https://doi.org/10.2478/aiht-2019-70-3322
Vancouver
Murati T, Miletić M, Kolarić J, Lovrić V, Bursać Kovačević D, Putnik P i sur. Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells. Arh Hig Rada Toksikol. [Internet]. 2019 [pristupljeno 15.08.2020.];70(4):303-308. https://doi.org/10.2478/aiht-2019-70-3322
IEEE
T. Murati, et al., "Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells", Arhiv za higijenu rada i toksikologiju, vol.70, br. 4, str. 303-308, 2019. [Online]. https://doi.org/10.2478/aiht-2019-70-3322

Sažetak
Prunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10-200 μg/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24, 48, and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 μg/mL (p<0.001–0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/ prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma.

Ključne riječi
blackthorn; cytotoxicity; Hep G2; kenacid blue; neutral red; ROS

Hrčak ID: 229324

URI
https://hrcak.srce.hr/229324

[hrvatski]

Posjeta: 132 *