Izvorni znanstveni članak
https://doi.org/10.15567/mljekarstvo.2020.0104
Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk
Harun Hizlisoy
orcid.org/0000-0003-3391-0185
; Erciyes University, Faculty of Veterinary Medicine, Department of Veterinary Public Health, 38039-Kayseri, Turkey
Nurhan Ertas Onmaz
; Erciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, Turkey
Serhat Al
orcid.org/0000-0003-2721-9275
; Erciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, Turkey
Fulden Karadal
orcid.org/0000-0002-5113-5883
; Omer Halisdemir University, Bor Vocational School, Department of Food Processing, Dairy Products and Technology, 51700 Nigde, Turkey
Yeliz Yildirim
; Erciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, Turkey
Zafer Gonulalan
; Erciyes University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, 38039-Kayseri, Turkey
K. Semih Gumussoy
; Erciyes University, Faculty of Veterinary Medicine, Department of Microbiology, 38039-Kayseri, Turkey
Gonca Demir Aydemir
; Erciyes University, Faculty of Medicine, Department of Medical Microbiology, 38039-Kayseri, Turkey
Filiz Kasap Tekinsen
; Erciyes University, Faculty of Medicine, Department of Medical Microbiology, 38039-Kayseri, Turkey
Gokcen Dinc
orcid.org/0000-0002-9184-5003
; Erciyes University, Faculty of Medicine, Department of Medical Microbiology, 38039-Kayseri, Turkey
Sažetak
The aim of this study was the isolation, identification, phylogenetic analysis and antifungal susceptibility of Candida spp. from milk samples of healthy and mastitic cows in Kayseri/Turkey. Milk samples from 300 cows were found to be negative/positive for mastitis with the California Mastitis Test. Candida spp. was isolated by using the Brillance Candida Agar Base. Phenotypic tests, Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF), and VITEK-2 analyses were applied to confirm the obtained isolates. Resistance to flucytosine, fluconazole and caspofungin antifungals of the isolates were determined by Etest and VITEK-2. The genetic homologies of Candida spp. isolates were determined by Repetitive Extragenic Palindromic-PCR (RepPCR). In this study 62 (from 53 healthy, 9 mastitic) yeast isolates were obtained and 37 (59.6 %) were identified as non-albicans Candida (NAC) species. Phenotypic tests revealed that out of 62 isolates, 29 (46.7 %), 4 (6.5 %), 3 (4.8 %), 1 (1.6 %) and, 24 (38.7 %) were identified as Candida lusitaniae, Candida catenulate, Candida tropicalis, Candida silvicola and other yeast species, respectively. Only one sample (1.6 %) was identified as Candida albicans by MALDI-TOF however, according to VITEK-2, the agent was not confirmed as C. albicans. According to antifungal susceptibility testing by VITEK-2, one (2.7 %) of the isolates was resistant to fluconazole, one (2.7 %) was resistant to caspofungin, and 4 (10.8 %) were resistant to flucytosine. However, using E test, 10 isolates (27 %) were resistant to flucytosine. Using Rep-PCR, eight genotypic clones were observed. Genotype F (13.8 %) and G (13.8 %; 2 subtypes) were common clones in this study. In conclusion, NAC species were detected in healthy and mastitic cow milk samples. Epidemiological studies need to be conducted to track effectively the main source and to understand the diversity and distribution of the agent. It is necessary to consider the potential risks of yeast contamination in milk for public health. It is essential to focus on adequate sanitation procedures and storage conditions of milk.
Ključne riječi
antifungal resistance; MALDI-TOF; VITEK-2; molecular typing; milk; non-albicans Candida (NAC) species
Hrčak ID:
231952
URI
Datum izdavanja:
8.1.2020.
Posjeta: 2.137 *