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https://doi.org/10.2478/acph-2021-0003

Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes

NATAŠA ZORIĆ ; Agency for Medicinal Products and Medical Devices of Croatia (HALMED), 10000 Zagreb, Croatia
NEVENKA KOPJAR ; Institute for Medical Research and Occupational Health, 10000 Zagreb, Croatia
JADRANKA VUKOVIĆ RODRIGUEZ ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis10000 Zagreb, Croatia
SINIŠA TOMIĆ ; Agency for Medicinal Products and Medical Devices of Croatia (HALMED), 10000 Zagreb, Croatia
IVAN KOSALEC ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Microbiology, 10000 Zagreb, Croatia

Puni tekst: engleski, pdf (457 KB) str. 131-141 preuzimanja: 120* citiraj
APA 6th Edition
ZORIĆ, N., KOPJAR, N., VUKOVIĆ RODRIGUEZ, J., TOMIĆ, S. i KOSALEC, I. (2021). Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes. Acta Pharmaceutica, 71 (1), 131-141. https://doi.org/10.2478/acph-2021-0003
MLA 8th Edition
ZORIĆ, NATAŠA, et al. "Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes." Acta Pharmaceutica, vol. 71, br. 1, 2021, str. 131-141. https://doi.org/10.2478/acph-2021-0003. Citirano 24.11.2020.
Chicago 17th Edition
ZORIĆ, NATAŠA, NEVENKA KOPJAR, JADRANKA VUKOVIĆ RODRIGUEZ, SINIŠA TOMIĆ i IVAN KOSALEC. "Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes." Acta Pharmaceutica 71, br. 1 (2021): 131-141. https://doi.org/10.2478/acph-2021-0003
Harvard
ZORIĆ, N., et al. (2021). 'Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes', Acta Pharmaceutica, 71(1), str. 131-141. https://doi.org/10.2478/acph-2021-0003
Vancouver
ZORIĆ N, KOPJAR N, VUKOVIĆ RODRIGUEZ J, TOMIĆ S, KOSALEC I. Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes. Acta Pharm. [Internet]. 2021 [pristupljeno 24.11.2020.];71(1):131-141. https://doi.org/10.2478/acph-2021-0003
IEEE
N. ZORIĆ, N. KOPJAR, J. VUKOVIĆ RODRIGUEZ, S. TOMIĆ i I. KOSALEC, "Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes", Acta Pharmaceutica, vol.71, br. 1, str. 131-141, 2021. [Online]. https://doi.org/10.2478/acph-2021-0003

Sažetak
This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 µmol L–1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy.

Ključne riječi
oleuropein; hydroxytyrosol; olive; antioxidant activity; DNA damage; human lymphocytes; comet assay

Hrčak ID: 236061

URI
https://hrcak.srce.hr/236061

Posjeta: 218 *