Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2020-71-3344
Cytotoxic and mutagenic potential of juglone: a comparison of free and nano-encapsulated form
Semiha Erisen
; Yildiz Technical University, Faculty of Science and Letters Molecular Biology and Genetics Department, Esenler- Istanbul, Turkey
Tülin Arasoğlu
; Yildiz Technical University, Faculty of Science and Letters Molecular Biology and Genetics Department, Esenler- Istanbul, Turkey
Banu Mansuroglu
; Yildiz Technical University, Faculty of Science and Letters Molecular Biology and Genetics Department, Esenler- Istanbul, Turkey
İsmail Kocacaliskan
; Yildiz Technical University, Faculty of Science and Letters Molecular Biology and Genetics Department, Esenler- Istanbul, Turkey
Serap Derman
; Yildiz Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Istanbul, Turkey
Sažetak
Despite its evidenced beneficial herbicidal, antibacterial, antiviral, antifungal, and antioxidant effects, the application of juglone (5-hydroxy-1,4,-naphthoquinone) is limited due to its low water solubility and allelopathic and toxic effects. In recent years, research has aimed to overcome these limitations by increasing its solubility and controlling its release through nanoparticular systems. This is the first study to have synthesised and characterised juglone-loaded polymeric nanoparticles and compared them with free juglone for cytotoxicity in mouse (L929 fibroblasts) and alfalfa cells and for mutagenic potential in Salmonella typhimurium TA98/100. Mouse and plant cells treated with free and nano-encapsulated juglone showed a decrease in cell viability in a dose and time-dependent manner, but this effect was significantly lower with the nanoencapsulated form at lower doses. In the TA98 strain with S9, nano-encapsulated juglone did not exhibit mutagenic effects, unlike the free form. Since all results show that juglone encapsulation with polymeric nanoparticles reduced the toxic and mutagenic effects, it has a promising potential to be applied in medicine, food safety, and agriculture.
Ključne riječi
alfalfa; L929 fibroblasts; nanoparticle; PLGA; S. typhimurium
Hrčak ID:
236214
URI
Datum izdavanja:
27.3.2020.
Posjeta: 1.184 *