Izvorni znanstveni članak
A comparison of the sensitivity of antibiotic residue screening methods - The Four Plate Test (FTP), The Screening test for antibiotic residues (STAR), and The Premi(R) Test to sulphonamide standards
Jana Janošová
; University of Veterinary Medcine, Komenského 73, 041 81 Košice, The SLovak Republic
Ivona Kožárová
; University of Veterinary Medcine, Komenského 73, 041 81 Košice, The Slovak Republic
Dionýz Máté
; University of Veterinary Medcine, Komenského 73, 041 81 Košice, The Slovak Republic
Miroslava Kovaliková
; University of Veterinary Medcine, Komenského 73, 041 81 Košice, The Slovak Republic
Sažetak
The sensitivity of three microbiological antibiotic residue screening methods, the FPT, the STAR and the Premi®Test, were compared for the detection of 10 different standards from the sulphonamide (SA) group. Phtalylsulphathiazole (PHT), sulphadimidine (SD), sulphaguanidine (SG), sulphachlorpyridazine (SCHP), sulphamerazine (SRZ), sulphamethoxazole (SMX), sulphanilamid (SAM), sulphanilic acid (SAC), sulphaquinoxaline (SQ) and sulphathiazole (STZ) were tested using the concentrations from 0.05 μg.ml-1 to 1 μg.ml-1. The detection sensitivity of the methods represented by minimum inhibiting concentration (MIC) was evaluated. The MIC of SA standards detected by FPT was 0.1 μg.ml-1 for SRZ, 0.2 μg.ml-1 for SMX, SQ and STZ, 0.3 μg.ml-1 for SCHP, and 1 μg.ml-1 for SD. No detection sensitivity was observed for PHT, SG, SAC and SAM standards. The MIC of SA standards detected by STAR method was 0.05 μg.ml-1 for SCHP, SMX, SQ and STZ, 0.1 μg.ml-1 for SRZ, and 0.3 μg.ml-1 for SD. No detection sensitivity was observed for PHT, SG, SAC and SAM standards. The MIC of SA standards detected by Premi®Test was 0.05 μg.ml-1 for SD, SCHP, SMX, SQ and STZ, 0.1 μg.ml-1 for SRZ and PHT, and 0.3 μg.ml-1 for SG, SAC and SAM. The MICs represent the detection limit of the methods (LOD). The results of examinations showed that Premi®Test is the most sensitive method to sulphonamides followed by STAR method and FPT. Premi®Test detected six SA at the level of the maximum residue limit (MRL) 0.1 μg.ml-1 set for SA group, STAR method detected
five SA at the level of MRL, and FPT detected only one SA at the level of MRL.
Ključne riječi
detection; FPT; STAR; Premi®Test; sulphonamides
Hrčak ID:
22062
URI
Datum izdavanja:
15.2.2007.
Posjeta: 2.179 *