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Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2020-71-3360

Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats

Naciye Dilara Zeybek   ORCID icon orcid.org/0000-0002-6161-5661 ; Hacettepe University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey
Ünzile Sur   ORCID icon orcid.org/0000-0002-4493-3684 ; 2 Hacettepe University Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey 3 Atatürk University Faculty of Pharmacy, Department of Toxicology, Erzurum, Turkey
Ofcan Oflaz ; Hacettepe University Faculty of Science, Department of Biology, Ankara, Turkey
Pınar Erkekoğlu ; Hacettepe University Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey
Aylin Balcı   ORCID icon orcid.org/0000-0002-0060-271X ; Hacettepe University Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey
Gizem Özkemahlı ; Erzincan Binali Yıldırım University Faculty of Pharmacy, Department of Toxicology, Erzincan, Turkey
Ali Aşçı ; Atatürk University Faculty of Pharmacy, Department of Toxicology, Erzurum, Turkey
Murat Kızılgün ; Dışkapı Children’s Health and Diseases, Haematology, and Oncology Training and Research Hospital, Ankara, Turkey
Oğuz Han Edebal ; Dışkapı Children’s Health and Diseases, Haematology, and Oncology Training and Research Hospital, Ankara, Turkey
Belma Koçer-Gümüşel   ORCID icon orcid.org/0000-0003-4311-2291 ; Hacettepe University Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey. Lokman Hekim University Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey

Puni tekst: engleski, pdf (3 MB) str. 110-120 preuzimanja: 65* citiraj
APA 6th Edition
Zeybek, N.D., Sur, Ü., Oflaz, O., Erkekoğlu, P., Balcı, A., Özkemahlı, G., ... Koçer-Gümüşel, B. (2020). Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats. Arhiv za higijenu rada i toksikologiju, 71 (2), 110-120. https://doi.org/10.2478/aiht-2020-71-3360
MLA 8th Edition
Zeybek, Naciye Dilara, et al. "Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats." Arhiv za higijenu rada i toksikologiju, vol. 71, br. 2, 2020, str. 110-120. https://doi.org/10.2478/aiht-2020-71-3360. Citirano 25.11.2020.
Chicago 17th Edition
Zeybek, Naciye Dilara, Ünzile Sur, Ofcan Oflaz, Pınar Erkekoğlu, Aylin Balcı, Gizem Özkemahlı, Ali Aşçı, Murat Kızılgün, Oğuz Han Edebal i Belma Koçer-Gümüşel. "Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats." Arhiv za higijenu rada i toksikologiju 71, br. 2 (2020): 110-120. https://doi.org/10.2478/aiht-2020-71-3360
Harvard
Zeybek, N.D., et al. (2020). 'Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats', Arhiv za higijenu rada i toksikologiju, 71(2), str. 110-120. https://doi.org/10.2478/aiht-2020-71-3360
Vancouver
Zeybek ND, Sur Ü, Oflaz O, Erkekoğlu P, Balcı A, Özkemahlı G i sur. Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats. Arh Hig Rada Toksikol. [Internet]. 2020 [pristupljeno 25.11.2020.];71(2):110-120. https://doi.org/10.2478/aiht-2020-71-3360
IEEE
N.D. Zeybek, et al., "Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and seleniumsupplemented rats", Arhiv za higijenu rada i toksikologiju, vol.71, br. 2, str. 110-120, 2020. [Online]. https://doi.org/10.2478/aiht-2020-71-3360

Sažetak
Aroclor 1254 (A1254), a mixture of polychlorinated biphenyls, exerts hepatic, renal, and reproductive toxicity in rodents. This study aimed to determine a protective role of selenium on histopathological changes, oxidative stress, and apoptosis caused by A1254 in rat kidney. It included a control group, which received regular diet containing 0.15 mg/kg Se (C), a Se-supplemented group (SeS) receiving 1 mg/kg Se, a Se-deficient group (SeD) receiving Se-deficient diet of ≤0.05 mg/ kg Se, an A1254-treated group (A) receiving 10 mg/kg of Aroclor 1254 and regular diet, an A1254-treated group receiving Se-supplementation (ASeS), and an A1254-treated group receiving Se-deficient diet (ASeD). Treatments lasted 15 days. After 24 h of the last dose of A1254, the animals were decapitated under anaesthesia and their renal antioxidant enzyme activities, lipid peroxidation (LP), glutathione, protein oxidation, and total antioxidant capacity levels measured. Histopathological changes were evaluated by light and electron microscopy. Apoptosis was detected with the TUNEL assay. Kidney weights, CAT activities, and GSH levels decreased significantly in all A1254-treated groups. Renal atrophic changes and higher apoptotic cell counts were observed in the A and ASeD groups. Both groups also showed a significant drop in GPx1 activities (A – 34.92 % and ASeD – 86.46 %) and rise in LP (A – 30.45 % and ASeD – 20.44 %) vs control. In contrast, LP levels and apoptotic cell counts were significantly lower in the ASeS group vs the A group. Histopathological changes and renal apoptosis were particularly visible in the ASeD group. Our findings suggest that selenium supplementation provides partial protection against renal toxicity of Aroclor 1254.

Ključne riječi
antioxidant enzymes; electron microscopy; histopathology; kidney; oxidative stress; polychlorinated biphenyls; Sprague-Dawley rats; TUNEL assay; ultrastructural changes

Hrčak ID: 239238

URI
https://hrcak.srce.hr/239238

[hrvatski]

Posjeta: 140 *