Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/21.2.2520
Biodiversity and symbiotic efficiency of indigenous rhizobia nodulating field pea
Mihaela BLAŽINKOV
; College of Slavonski Brod, Dr. M. Budaka 1, 35000 Slavonski Brod, Croatia
Darko UHER
; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Nataša ROMANJEK FAJDETIĆ
; College of Slavonski Brod, Dr. M. Budaka 1, 35000 Slavonski Brod, Croatia
Božica JAPUNDŽIĆ –PALENKIĆ
; College of Slavonski Brod, Dr. M. Budaka 1, 35000 Slavonski Brod, Croatia
Sanja SIKORA
; University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Sažetak
The main aim of this study was to assess genetic diversity within the natural populations of field pea rhizobia on different field sites in Northwestern Croatia and to evaluate their symbiotic efficiency. Identification of related bacterial strains was carried out using RAPD and rep-PCR methods and on the basis of differences in the nodulation nodD gene region by PCR-RFLP method. Indigenous strains have been shown to differ significantly from each other as well as from the reference strains Rhizobium leguminosarum bv. viciae used in these study. Based on the nodulation genes, it was found that most isolates have the same or very similar nodulation nodD region except the isolates K22 and K23. A greenhouse studie was performed for evaluation of symbiotic efficiency of strains. The highest nodule dry weight was determined by inoculation with strains K23, K22, K17 and K20, indicating their high infectivity and nodulation ability. Significantly higher green mass and dry matter yield in abouveground plant parts were determined by inoculation with
two indigenous strains K26 and K16 that showed potentially high symbiotic efficiency compared to other tested strains.
Ključne riječi
biological nitrogen fixation; rhizobia; field pea; genetic diversity
Hrčak ID:
239368
URI
Datum izdavanja:
18.6.2020.
Posjeta: 811 *