Izvorni znanstveni članak

https://doi.org/10.3325/cmj.2019.60.71

Brain derived neurotrophic factor expression and DNA methylation in response to subchronic valproic acid and/or aldosterone treatment

Katarina Buzgoova ; Laboratory of Pharmacological Neuroendocrinology, Instituteof Experimental EndocrinologyBiomedical Research Center, SlovakAcademy of Sciences, Bratislava, Slovakia
Jan Graban ; Laboratory of Pharmacological Neuroendocrinology, Instituteof Experimental EndocrinologyBiomedical Research Center, SlovakAcademy of Sciences, Bratislava, Slovakia
Lucia Balagova ; Laboratory of Pharmacological Neuroendocrinology, Instituteof Experimental EndocrinologyBiomedical Research Center, SlovakAcademy of Sciences, Bratislava, Slovakia
Natasa Hlavacova ; Laboratory of Pharmacological Neuroendocrinology, Instituteof Experimental EndocrinologyBiomedical Research Center, SlovakAcademy of Sciences, Bratislava, Slovakia
Daniela Jezova ; Laboratory of Pharmacological Neuroendocrinology, Instituteof Experimental EndocrinologyBiomedical Research Center, SlovakAcademy of Sciences, Bratislava, Slovakia

Sažetak

Aim To test the hypothesis that valproic acid treatment
positively affects brain-derived neurotrophic factor (BDNF)
expression and DNA methylation in the hippocampus and
brain cortex of rats simultaneously treated with aldosterone.
Methods Male Sprague-Dawley rats (N = 40) were treated
for two weeks with valproic acid (100 mg/1 kg body
weight/d) in drinking water and aldosterone (2 μg/100 g
body weight/d) or placebo via subcutaneous osmotic minipumps. Results Treatment with valproic acid did not modify BDNF
gene expression in the hippocampus but reduced BDNF
mRNA levels in the brain cortex. Valproic acid treatment
marginally enhanced global DNA methylation in the frontal
cortex. BDNF expression negatively correlated with
DNA methylation in the hippocampus of valproic acidtreated
rats. An unexpected finding was that aldosterone
treatment significantly decreased global DNA methylation
in the hippocampus.
Conclusion The effect of valproic acid on BDNF expression
in the brain may depend on the extent of pathological
changes present at the time of treatment onset. The
observed negative correlation between BDNF expression
and DNA methylation in the hippocampus of valproic acid-
treated rats encourages further studies.

Ključne riječi

Hrčak ID:

239733

URI

https://hrcak.srce.hr/239733

Datum izdavanja:

15.4.2019.

Posjeta: 564 *