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https://doi.org/10.37427/botcro-2020-024

Influence of dehydration on cryopreservation of Musa spp. germplasm

Ergun Kaya   ORCID icon orcid.org/0000-0003-4255-3802 ; Mugla Sitki Kocman University, Faculty of Science, Molecular Biology and Genetics Department, 48000 Kotekli, Mugla, Turkey
Fernanda Vidigal Duarte Souza ; Embrapa Cassava and Fruits, Caixa Postal 007, Cruz das Almas, BA 44380-000, Brazil
Janay Almeida dos Santos-Serejo ; Embrapa Cassava and Fruits, Caixa Postal 007, Cruz das Almas, BA 44380-000, Brazil
Selin Galatali ; Mugla Sitki Kocman University, Faculty of Science, Molecular Biology and Genetics Department, 48000 Kotekli, Mugla, Turkey

Puni tekst: engleski, pdf (932 KB) str. 99-104 preuzimanja: 240* citiraj
APA 6th Edition
Kaya, E., Vidigal Duarte Souza, F., Almeida dos Santos-Serejo, J. i Galatali, S. (2020). Influence of dehydration on cryopreservation of Musa spp. germplasm. Acta Botanica Croatica, 79 (2), 99-104. https://doi.org/10.37427/botcro-2020-024
MLA 8th Edition
Kaya, Ergun, et al. "Influence of dehydration on cryopreservation of Musa spp. germplasm." Acta Botanica Croatica, vol. 79, br. 2, 2020, str. 99-104. https://doi.org/10.37427/botcro-2020-024. Citirano 27.10.2021.
Chicago 17th Edition
Kaya, Ergun, Fernanda Vidigal Duarte Souza, Janay Almeida dos Santos-Serejo i Selin Galatali. "Influence of dehydration on cryopreservation of Musa spp. germplasm." Acta Botanica Croatica 79, br. 2 (2020): 99-104. https://doi.org/10.37427/botcro-2020-024
Harvard
Kaya, E., et al. (2020). 'Influence of dehydration on cryopreservation of Musa spp. germplasm', Acta Botanica Croatica, 79(2), str. 99-104. https://doi.org/10.37427/botcro-2020-024
Vancouver
Kaya E, Vidigal Duarte Souza F, Almeida dos Santos-Serejo J, Galatali S. Influence of dehydration on cryopreservation of Musa spp. germplasm. Acta Botanica Croatica [Internet]. 2020 [pristupljeno 27.10.2021.];79(2):99-104. https://doi.org/10.37427/botcro-2020-024
IEEE
E. Kaya, F. Vidigal Duarte Souza, J. Almeida dos Santos-Serejo i S. Galatali, "Influence of dehydration on cryopreservation of Musa spp. germplasm", Acta Botanica Croatica, vol.79, br. 2, str. 99-104, 2020. [Online]. https://doi.org/10.37427/botcro-2020-024

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Sažetak
Cryopreservation is an important technique for the long-term storage of economically important plant germplasm. In this study, an efficient protocol was developed for the long-term conservation of seven economically important Musa taxa: M. acuminata Colla ssp. burmannica N.W. Simmonds, M. acuminata Colla ssp. zebrina (Van Houtte) R.E. Nasution, M. balbisiana Colla, M. basjoo Sieb., M. ornata W. Roxburgh (St. Lavender), M. velutina H. Wendl. et Drude (Velvet Pink Banana), and M. acuminata’ balbisiana. The seeds were dehydrated in a sterile laminar flow cabinet for different exposure times and then they were directly immersed in liquid nitrogen. The critical point was to support the initial germination of cryopreserved seeds and this was achieved by the excision of zygotic embryos after liquid nitrogen treatment that allowed the seed germination. The best moisture content for tolerance to cryopreservation ranged from 15.8% (M. acuminata ssp. zebrina) to 17.1% (M. ornata) and the maximum post-cryopreservation germination rates varied from 86.4% (M. velutina) to 55.0% (M. ornata). All seedlings derived from seeds germinated after cryopreservation were easily rooted and acclimated to greenhouse conditions.

Ključne riječi
banana; long-term conservation; moisture content; seed desiccation

Hrčak ID: 240724

URI
https://hrcak.srce.hr/240724

Posjeta: 530 *