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https://doi.org/10.18054/pb.v121-122i3-4.11132

Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells

Antonio Periš
Marijana Jukić   ORCID icon orcid.org/0000-0002-3326-4868
Luka Krstulović
Miro Bajić
Ljubica Glavaš-Obrovac   ORCID icon orcid.org/0000-0001-7497-296X ; School of Medicine, University Josip Juraj Strossmayer of Osijek

Puni tekst: engleski, pdf (704 KB) str. 161-167 preuzimanja: 48* citiraj
APA 6th Edition
Periš, A., Jukić, M., Krstulović, L., Bajić, M. i Glavaš-Obrovac, Lj. (2020). Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells. Periodicum biologorum, 121-122 (3-4), 161-167. https://doi.org/10.18054/pb.v121-122i3-4.11132
MLA 8th Edition
Periš, Antonio, et al. "Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells." Periodicum biologorum, vol. 121-122, br. 3-4, 2020, str. 161-167. https://doi.org/10.18054/pb.v121-122i3-4.11132. Citirano 17.05.2021.
Chicago 17th Edition
Periš, Antonio, Marijana Jukić, Luka Krstulović, Miro Bajić i Ljubica Glavaš-Obrovac. "Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells." Periodicum biologorum 121-122, br. 3-4 (2020): 161-167. https://doi.org/10.18054/pb.v121-122i3-4.11132
Harvard
Periš, A., et al. (2020). 'Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells', Periodicum biologorum, 121-122(3-4), str. 161-167. https://doi.org/10.18054/pb.v121-122i3-4.11132
Vancouver
Periš A, Jukić M, Krstulović L, Bajić M, Glavaš-Obrovac Lj. Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells. Periodicum biologorum [Internet]. 2020 [pristupljeno 17.05.2021.];121-122(3-4):161-167. https://doi.org/10.18054/pb.v121-122i3-4.11132
IEEE
A. Periš, M. Jukić, L. Krstulović, M. Bajić i Lj. Glavaš-Obrovac, "Effects of quinoline-arylamidine hybrids on LPS-induced inflammation in RAW 264.7 cells", Periodicum biologorum, vol.121-122, br. 3-4, str. 161-167, 2020. [Online]. https://doi.org/10.18054/pb.v121-122i3-4.11132

Sažetak

Background and purpose: Inflammation is a common pathogenesis in infection, injury, cancer, and many chronic diseases. Macrophages are among the main cells involved in generation of inflammation. The aim of the present study was to investigate the effects of molecular hybrids with 7-chloroquinoline and arylamidine moieties joined through flexible a 2-aminoethanol linker, on the in vitro inflammatory responses to lipopolysaccharides (LPS) induced inflammation in the RAW 264.7 cells.


Materials and methods: To determine effects of seven quinoline-arylamidine hybrids on the growth of the murine macrophage-like (RAW 264.7) cells MTT assay was used. Inflammatory reactions in the RAW264.7 cells were induced using E. coli lipopolysaccharides (LPS). Levels of nitric oxide (NO) and malondialdehyde (MDA) were determined by spectrophotometry methods. Intracellular production of reactive oxygen species (ROS) was measured by flow cytometry. Antioxidant capacity of tested compounds was tested by 2,2'-azino-bis(3-ethybenzthiazoline-6-sulfonic acid (ABTS) radical cation method.


Results: Tested hybrid compounds differentially influenced proliferation of non-stimulated and LPS-stimulated RAW 264.7 cells. The hybrid compounds have not presented ABTS radical-scavenger activity. In the LPS-stimulated RAW 264.7 cells 10 μM compounds slightly decreased production of NO and ROS and significantly modulated LPS-induced lipid peroxidation.


Conclusions: Molecular hybrids with 7-chloroquinoline and arylamidine moieties joined through flexible 2-aminoethanol linker markedly decreased accumulation of lipid peroxidation products in the LPS-stimulated RAW 264.7 cells. Further studies are necessary to determine their mechanism of anti-inflammatory action in more details.


 


Keywords: Hybrid molecules, 7-Chloroquinoline, Aromatic amidine, Anti-inflammatory activity in vitro

Hrčak ID: 254658

URI
https://hrcak.srce.hr/254658

Posjeta: 89 *